Protein Info for Pf6N2E2_4013 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): 5-aminovalerate transaminase (EC 2.6.1.48)
Rationale: Specifically important for utilizing L-Lysine. Automated validation from mutant phenotype: the predicted function (2.6.1.48) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: 5-aminovalerate aminotransferase (EC 2.6.1.48) / Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00700: 4-aminobutyrate transaminase
  • amino acids 11 to 422 (412 residues), 542.1 bits, E=3.7e-167
• PF00202: Aminotran_3
  • amino acids 17 to 421 (405 residues), 455.6 bits, E=1.3e-140
• PF00155: Aminotran_1_2
  • amino acids 61 to 412 (352 residues), 23 bits, E=4.3e-09

Best Hits

Swiss-Prot: 87% identical to DAVT_PSEPK: 5-aminovalerate aminotransferase DavT (davT) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a175)

MetaCyc: 97% identical to 4-aminobutyrate transaminase subunit (Pseudomonas fluorescens)
4-aminobutyrate transaminase. [EC: 2.6.1.19]

Predicted SEED Role

"5-aminovalerate aminotransferase (EC 2.6.1.48) / Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)" in subsystem Lysine degradation (EC 2.6.1.19, EC 2.6.1.48)

MetaCyc Pathways

• superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
• L-lysine degradation IV (5/5 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
• L-lysine degradation X (5/6 steps found)
• 4-aminobutanoate degradation II (2/2 steps found)
• 4-aminobutanoate degradation III (2/2 steps found)
• superpathway of 4-aminobutanoate degradation (2/3 steps found)
• 4-aminobutanoate degradation I (1/2 steps found)
• GABA shunt II (2/4 steps found)
• L-lysine degradation I (4/7 steps found)
• GABA shunt I (1/4 steps found)
• L-lysine degradation III (2/6 steps found)
• 4-aminobutanoate degradation V (2/7 steps found)
• nicotine degradation I (pyridine pathway) (5/17 steps found)
• superpathway of L-lysine degradation (19/43 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Butanoate metabolism
• Glutamate metabolism
• Lysine degradation
• Propanoate metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.19

Use Curated BLAST to search for 2.6.1.19 or 2.6.1.48

Sequence Analysis Tools

See A0A160A2Z7 at UniProt or InterPro

Protein Sequence (425 amino acids)

>Pf6N2E2_4013 5-aminovalerate transaminase (EC 2.6.1.48) (Pseudomonas fluorescens FW300-N2E2)
MSKTNADLMARRTAAVPRGVGQIHPIFAESAKNATVTDVEGREFIDFAGGIAVLNTGHVH
PKIIAAVTEQLNKLTHTCFQVLAYEPYVELCEKINAKVPGDFAKKTLLVTTGSEAVENAV
KIARAATGRAGVIAFTGAYHGRTMMTLGLTGKVVPYSAGMGLMPGGIFRALYPNELHGVS
IDDSIASIERIFKNDAEPRDIAAIIIEPVQGEGGFYVAPKEFMKRLRALCDQHGILLIAD
EVQTGAGRTGTFFAMEQMGVAADLTTFAKSIAGGFPLAGVCGKAEYMDAIAPGGLGGTYA
GSPIACAAALAVMEVFEEEHLLDRCKAVGERLVAGLKAIQKKYPVIGDVRALGAMIAVEL
FENGDSHKPNAAAVAQVVAKARDKGLILLSCGTYGNVLRVLVPLTAPDEQLDKGLAILEE
CFSEL