Protein Info for Pf6N2E2_401 in Pseudomonas fluorescens FW300-N2E2

Annotation: Cyn operon transcriptional activator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 299 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF00126: HTH_1" amino acids 8 to 65 (58 residues), 87.8 bits, see alignment E=3.5e-29 PF03466: LysR_substrate" amino acids 92 to 293 (202 residues), 171.9 bits, see alignment E=1.2e-54

Best Hits

Swiss-Prot: 63% identical to CYNR_ECOLI: HTH-type transcriptional regulator CynR (cynR) from Escherichia coli (strain K12)

KEGG orthology group: K11921, LysR family transcriptional regulator (inferred from 85% identity to ppw:PputW619_3094)

Predicted SEED Role

"Cyn operon transcriptional activator" in subsystem Cyanate hydrolysis or DNA-binding regulatory proteins, strays

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZV35 at UniProt or InterPro

Protein Sequence (299 amino acids)

>Pf6N2E2_401 Cyn operon transcriptional activator (Pseudomonas fluorescens FW300-N2E2)
MVKTMLARHVQYFLAVAEHLSFTKAAAALHVSQPALSQQVRQLEESLGAQLFDRSGRTTR
LTDAGDVYLLYARRAYQQLREAKSAIHDVSDLTRGSLRLAVTPTFTTYLVGPVIKAFHSR
YPKITLNLKEISQERIEELLLAGELDAGIAFDEINTPNIDAIPLLNETLALVVSRQHRLA
EERSIGLQDLNAESLILLSPEFATREQIDRYFRTHAIHPQVQMEANALGAVIEIVRRTNL
STLLPAKIALAHDDLVAIALEPERLQRTAVLMRRKDAFQSAAMRVFIEVAKEVAIQLNV