Protein Info for Pf6N2E2_4009 in Pseudomonas fluorescens FW300-N2E2

Annotation: GGDEF domain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 635 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 43 to 62 (20 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 80 to 204 (125 residues), 57.3 bits, see alignment E=1.8e-19 PF00989: PAS" amino acids 90 to 194 (105 residues), 41.2 bits, see alignment E=5.2e-14 PF08448: PAS_4" amino acids 92 to 196 (105 residues), 23.5 bits, see alignment E=2e-08 PF13426: PAS_9" amino acids 94 to 195 (102 residues), 61 bits, see alignment E=4.2e-20 PF08447: PAS_3" amino acids 105 to 190 (86 residues), 31.3 bits, see alignment E=6.9e-11 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 205 to 367 (163 residues), 129 bits, see alignment E=1.4e-41 PF00990: GGDEF" amino acids 208 to 364 (157 residues), 153.7 bits, see alignment E=1.3e-48 PF00563: EAL" amino acids 386 to 620 (235 residues), 255.8 bits, see alignment E=1.2e-79

Best Hits

KEGG orthology group: None (inferred from 96% identity to pba:PSEBR_a179)

Predicted SEED Role

"GGDEF domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A0Q5 at UniProt or InterPro

Protein Sequence (635 amino acids)

>Pf6N2E2_4009 GGDEF domain protein (Pseudomonas fluorescens FW300-N2E2)
MPAYQRSAMRVALLYLVLSVVWLQLIGYLLSSFFDQFADRQRWLLINGYAWVLLSAGLIF
LARARISRFFAKGEHGADRERLRQAAAVFDCTREGVLVTDRNGLIVHVNRAFMAITGYVR
DEVLGQRPSLFKSGRHGPDFYRDMFTSLDSQGEWSGEIWNRRKSGEIYPQWQTIRVIRDD
SGEISHYVAVFSDISAIKDSEHELAYLAHHDPLTGLPNRLLFSDRTEQALASAQLHKRGC
ALLLLDLDHFKNINDSLGHNVGDELLKGVAERFRGLFAPGVTLARLGGDEFAVLVENCSQ
PGQAAALARRILDALKEPLRFDDHALFINASIGISLFPGDALSAGQLLRNADSALFKAKS
AGRDGYALYTEELTAHAQQRVEIAFELRRALQQQELRVHYQPVHDLATSRLIGVEALVRW
EHPTRGLVSPAEFIPIAERTGLIAQVDAWVMDQACRQMCQWQQAGVALSFVAVNVSSRLF
ARRELYDQVAQVLHDTGLDPGCLELEVTESAVMDDPEVALEQMHRLRELGVRLAIDDFGT
GYSSLLRLKRLPVQKLKIDQGFVAGLPWDEDDAAIVRVIIALAHSMGMQVHAEGIEQREQ
AAFLLGQACELGQGYWFGRPVAAAQLDWHCAPVIA