Protein Info for Pf6N2E2_4006 in Pseudomonas fluorescens FW300-N2E2

Annotation: Sulfate and thiosulfate binding protein CysP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 337 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR00971: sulfate ABC transporter, sulfate-binding protein" amino acids 18 to 333 (316 residues), 464.2 bits, see alignment E=1.1e-143 PF01547: SBP_bac_1" amino acids 42 to 279 (238 residues), 58.5 bits, see alignment E=1.8e-19 PF13531: SBP_bac_11" amino acids 48 to 287 (240 residues), 126.1 bits, see alignment E=2.9e-40 PF13343: SBP_bac_6" amino acids 128 to 296 (169 residues), 25.5 bits, see alignment E=1.4e-09

Best Hits

Swiss-Prot: 66% identical to SUBI_ECOLI: Sulfate-binding protein (sbp) from Escherichia coli (strain K12)

KEGG orthology group: K02048, sulfate transport system substrate-binding protein (inferred from 99% identity to pba:PSEBR_a182)

MetaCyc: 66% identical to sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate and thiosulfate binding protein CysP" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A1V6 at UniProt or InterPro

Protein Sequence (337 amino acids)

>Pf6N2E2_4006 Sulfate and thiosulfate binding protein CysP (Pseudomonas fluorescens FW300-N2E2)
MSSIRRYALAALASAVFAGSAVAKDYELLNVSYDPTRELYQDYNAEFASFWKKAHPDDSV
KIQQSHGGSGKQGRAVIDGLRADVVTLALAGDIDEIAKLGKSLPADWQTRLPEASTPYTS
TIVFLVRKGNPKGIKDWGDLIKNDVSVITPNPKTSGGARWNFLAAWAYGLKANGGDEAKA
KEYVQALFKHVPILDTGARGSTITFVNNGQGDVLLAWENEAFLALKEDGGADKFDIVVPS
LSILAEPPVAVVDKNAEKKGNAEIAEAYLKHLYSPAGQEIAAKNFYRPRDKDVAAKYSRQ
FPKLDLVTIDKDFGGWKTAQPKFFNDGGVFDQIYQAQ