Protein Info for Pf6N2E2_3948 in Pseudomonas fluorescens FW300-N2E2

Annotation: Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 739 transmembrane" amino acids 125 to 143 (19 residues), see Phobius details amino acids 150 to 168 (19 residues), see Phobius details amino acids 180 to 197 (18 residues), see Phobius details amino acids 202 to 220 (19 residues), see Phobius details amino acids 353 to 378 (26 residues), see Phobius details amino acids 384 to 410 (27 residues), see Phobius details amino acids 693 to 711 (19 residues), see Phobius details amino acids 716 to 737 (22 residues), see Phobius details TIGR01525: heavy metal translocating P-type ATPase" amino acids 182 to 736 (555 residues), 580.7 bits, see alignment E=7.9e-178 TIGR01494: HAD ATPase, P-type, family IC" amino acids 204 to 718 (515 residues), 261.7 bits, see alignment E=2e-81 PF00122: E1-E2_ATPase" amino acids 233 to 334 (102 residues), 90.9 bits, see alignment E=7.9e-30 PF00702: Hydrolase" amino acids 433 to 645 (213 residues), 123.6 bits, see alignment E=2.8e-39

Best Hits

KEGG orthology group: K01534, Cd2+/Zn2+-exporting ATPase [EC: 3.6.3.3 3.6.3.5] (inferred from 98% identity to pba:PSEBR_a240)

MetaCyc: 76% identical to cadmium translocating P-type ATPase (Pseudomonas putida KT2440)
3.6.3.3-RXN [EC: 7.2.2.21]

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5 or 7.2.2.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZYW7 at UniProt or InterPro

Protein Sequence (739 amino acids)

>Pf6N2E2_3948 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) (Pseudomonas fluorescens FW300-N2E2)
MSDSLHTHKPDHGHDHGHSCCASKAAPAVVILGKTPTDGARLSSFRIEAMDCPTEQTLIQ
NKLGKLEGVQRLDFNLINRVLGVTHDLPSDAPIIKAIESLGMQAEPMTPGKEKASPDLPA
PAKPWWPLALSGVTALGAELIHFTNAAPNWVVALVALVSILSGGLGTYKKGWIALKNRNL
NINALMSIAVTGAVLIGQWPEAAMVMFLFTVAELIEAKSLDRARNAISGLMQMAPQQATV
QQADGSWQVQDVKVIALGARIRLRPGERVGLDGDVVAGRSTIDQAPITGESLPVEKTVGD
KVFAGTINQAGELEYTVTAAADHSTLARIIHAVEQAQGSRAPTQRFVDQFSKIYTPAVFV
LALAVAIIPPLFMGAAWFDWIYRALVLLVVACPCALVISTPVTIVSGLAAAARKGILVKG
GVYLEGGYKLDYLALDKTGTLTHGKPVQTDYVALDPSVETTAPALAASLAARSDHPVSRA
IANAAVDKQLAQQVVDNFEALAGRGVRGDIGGQTYHLGNHRLVEDLGLCSPELEEKLFAL
EKQGKSVVLLLDATGPLALFAVADTVKDSSREAIRQLHDLGIKTLMLTGDNAHTADAIAA
QVGMDQARGDLLPEDKLQAIEALYAQGHRVGMVGDGINDAPALARSEIGFAMAAAGTDTA
IETADVALMDDDLRKIPAFIRLSRQTSSILKQNIALALVIKAIFLGFTFAGFATMWMAVF
ADMGVSLLVVFNGLRLLRK