Protein Info for Pf6N2E2_3917 in Pseudomonas fluorescens FW300-N2E2

Annotation: High-affinity choline uptake protein BetT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 667 transmembrane" amino acids 18 to 44 (27 residues), see Phobius details amino acids 57 to 79 (23 residues), see Phobius details amino acids 93 to 114 (22 residues), see Phobius details amino acids 148 to 168 (21 residues), see Phobius details amino acids 192 to 214 (23 residues), see Phobius details amino acids 234 to 255 (22 residues), see Phobius details amino acids 265 to 284 (20 residues), see Phobius details amino acids 322 to 340 (19 residues), see Phobius details amino acids 352 to 372 (21 residues), see Phobius details amino acids 411 to 436 (26 residues), see Phobius details amino acids 452 to 474 (23 residues), see Phobius details amino acids 480 to 499 (20 residues), see Phobius details PF02028: BCCT" amino acids 18 to 503 (486 residues), 601.5 bits, see alignment E=5.2e-185 TIGR00842: transporter, betaine/carnitine/choline transporter (BCCT) family" amino acids 56 to 503 (448 residues), 512.9 bits, see alignment E=3.7e-158

Best Hits

KEGG orthology group: K02168, high-affinity choline transport protein (inferred from 99% identity to pba:PSEBR_a270)

Predicted SEED Role

"High-affinity choline uptake protein BetT" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZYQ1 at UniProt or InterPro

Protein Sequence (667 amino acids)

>Pf6N2E2_3917 High-affinity choline uptake protein BetT (Pseudomonas fluorescens FW300-N2E2)
MSASSLPPSGLVRMNPPVFYFAATFILLFGLVVIAMPAQAGAWLLAAQNWAANTVGWYYM
LAMTLYLVFVVVTALSGYGKIKLGADHDEPEFSYLSWAGMLFAAGISITLFFFCVSEPLT
HMVQPPQGEAGGTAEAARQAMQILFLHWGLHGWGVFAFVGMALAYFAYRHNLPLALRSAL
YPLIGKRINGPIGYAVDGFGIIATVFGLGADMGFGVLHLNSGLDYLFGIAHTQWIQVGLI
VLMMGAAIIVAVAGVDKGVRVMSDINMLLACALLLFVLFAGPTQHLLNTLIQNIGDYLGA
LPMKSFDLYAYDKPSDWLGGWTVFYWAWWIAWSPFVGLFIARISRGRTIREFVFGVLLIP
LGFTLAWMSIFGNSAIDQVLNHGMSALGMSALENPSMSLYLLLETYPWSKTVIAVTVFIS
FVFFVTSADSGTVVLSTLSAKGGNPDEDGPKWLRVFWGAMTALVTSALLFSGSIDALKSA
VVLTSLPFSLILLLMMWGLHKAFYLESQKQIAQLHSLAPVSGSRRGTGGWRQRLSQAVHF
PSRDEVYRFLDTTVRPAIEEVTAVFVEKGLTVVTQPDPANDNVSLEIGHGEQHPFIYQVQ
MRGYFTPSFARGGMGSKELNNRRYYRAEVHLSEGSQDYDLVGYTKEQVINDILDQYERHL
QFLHLVR