Protein Info for Pf6N2E2_3908 in Pseudomonas fluorescens FW300-N2E2

Annotation: Glutamate--cysteine ligase (EC 6.3.2.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 532 transmembrane" amino acids 199 to 217 (19 residues), see Phobius details TIGR01434: glutamate--cysteine ligase" amino acids 12 to 521 (510 residues), 767.1 bits, see alignment E=3.9e-235 PF04262: Glu_cys_ligase" amino acids 15 to 386 (372 residues), 564.7 bits, see alignment E=3.6e-174

Best Hits

Swiss-Prot: 90% identical to GSH1_PSEPF: Glutamate--cysteine ligase (gshA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 98% identity to pba:PSEBR_a278)

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161H8P9 at UniProt or InterPro

Protein Sequence (532 amino acids)

>Pf6N2E2_3908 Glutamate--cysteine ligase (EC 6.3.2.2) (Pseudomonas fluorescens FW300-N2E2)
VKEFNLSDLLNRRLALLGERANLSLLEQCLHGIERECLRVTGDGHLAQTPHPKALGSALT
NEQITTDYSESLLEFITPALTKPADTLRSLDKIHRFAYSKLDDEYLWSPSMPCPLPAEED
IPIAYYGTSNIGRLKYVYRKGLALRYGKTMQCIAGIHYNFSLPEKLWPLLRQAEGVEASD
RDFQSSAYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRPHQLEQLDPDTLYLPYATSL
RMSDLGYQSNAQAGLTPCYNDLASYTDSLRKAVATPYAPYVEVGTHQNGEWVQLNTNILQ
IENEYYSNIRPKRVTYTGERPIQALMARGIQYVEVRCLDINPFLPMGIDLTESRFLDAFL
LYCGLNDSPQLENNECGNATSNFLTVVKEGRKPGLQLQRQGQAVDMQAWAVELLEKIAPL
AALLDQSQGGDAHRQALDAQLAKVRDPSLTPSAQVLAAMAEHQESFAQFSLRQSKAHAEF
FRAEPLPAQEQAAFEDAARESLVQQAELEQNEVGDFDVFVGSYQASILAISN