Protein Info for Pf6N2E2_3899 in Pseudomonas fluorescens FW300-N2E2

Annotation: Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 98% identical to PCKA_PSEF5: Phosphoenolpyruvate carboxykinase (ATP) (pckA) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K01610, phosphoenolpyruvate carboxykinase (ATP) [EC: 4.1.1.49] (inferred from 100% identity to pba:PSEBR_a287)

Predicted SEED Role

"Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP or Serine-glyoxylate cycle (EC 4.1.1.49)

MetaCyc Pathways

gluconeogenesis I (11/13 steps found)
superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
C4 photosynthetic carbon assimilation cycle, PEPCK type (9/14 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W7B1 at UniProt or InterPro

Protein Sequence (514 amino acids)

>Pf6N2E2_3899 Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) (Pseudomonas fluorescens FW300-N2E2)
MTQANNAVYTDLSVDDLVKEALNRGEGELADTGALVVRTGHRTGRSPVDRFIVEEPTTQD
AIAWGPINRKFPADKFDALWNRVEAYLGERERFVSHVHVGSDPAHYLPVKMTTETAWHNL
FGRCLFINPEQYNAGGKDEWQILNAPNFVCEPERDGTNSDGTVIINFAAKKVLIAGMRYA
GEMKKALFSVQNFLLPAVDVLPMHCAANMGEEGDVTLFFGLSGTGKTTLSADESRYLIGD
DEHGWGVGVVFNIEGGCYAKCIDLSEKNEPVIWKAIQHGAVLENVVLDPVTKKADYADDS
LTQNSRAAYPRELIEKRAPKNLGGEPNAVIFLTCDLTGVLPPVSILSEEQAAYHFLSGYT
ALVGSTEMGSGSGIKSTFSTCFGAPFFPRPAGEYAELLIKRIRGFGSKVYLVNTGWTGGG
YGVGKRFNIPTTRAVIAAIQSGALIGAETEHLDIINLDVPLAVPGVETGLLNPRNTWADK
AAYDEAAKALAGLFTENFKKFDVSDAIKAAGPQL