Protein Info for Pf6N2E2_3891 in Pseudomonas fluorescens FW300-N2E2

Annotation: Putative formate dehydrogenase oxidoreductase protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01701: oxidoreductase alpha (molybdopterin) subunit
  • amino acids 22 to 770 (749 residues), 957 bits, E=3.2e-292
• PF00384: Molybdopterin
  • amino acids 118 to 499 (382 residues), 99.8 bits, E=8.6e-33

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 99% identity to pba:PSEBR_a294)

Predicted SEED Role

"Putative formate dehydrogenase oxidoreductase protein" in subsystem Formate hydrogenase

MetaCyc Pathways

• formate oxidation to CO₂ (1/1 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• oxalate degradation VI (1/4 steps found)
• oxalate degradation III (1/5 steps found)
• superpathway of C1 compounds oxidation to CO₂ (5/12 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

See A0A160A1L7 at UniProt or InterPro

Protein Sequence (782 amino acids)

>Pf6N2E2_3891 Putative formate dehydrogenase oxidoreductase protein (Pseudomonas fluorescens FW300-N2E2)
VSNHQQADQKPVPRYKPYKGPAGGWGALISVAQAWLTSDNALKNIRMMLKTNQNGGFDCP
GCAWGDSPESGMVKFCENGAKAVNWEATKRRVDGAFFAKHSVSSLLEQSDYWLEYQGRLT
EPMRYDAETDRYKPVSWEAAFDLIGKHLQGLSSPNQAEFYTSGRASNEAAYLYQLFVRAY
GTNNFPDCSNMCHEASGVALSQSVGVGKGTVTFDDFEHADAIFVWGQNPGTNHPRMLEPL
REAVKRGAQVVCINPLKERGLERFQHPQNPIEMLTNGNKPTNTAYFRPALGGDMAILRGM
AKFLLQWEREAQNTGAAAVFDHAFLNEHSTNVLDYLAAVDDTPWEQIVEQSGMTLVDIEQ
AARMYAKSQNVIMCWAMGITQHRHSVATIQEIANVMMLRGNIGRPGAGLCPVRGHSNVQG
DRTMGINERPPTAFLDSLERRFQFKVPRENGHNVVEAIHAMASGEAKVFIALGGNFAQAT
PDSHRTFQALSNCDLTVQISTKLNRSHLAHGKEALILPCLGRTDIDLQTEGAQAVTVEDS
FSMVHASMGQLQPLSNQMRSEPWIIAGIAAATLGSRPVDWNWLVADYSRIRDLIADTVPG
FKDFNEKLKNPGGFYLGNSAGSRRWNTPSGRANFKPNRLPTDLVHERTRATGQLPDLIMQ
SMRSHDQYNTTIYGLDDRYRGVKGQRDVLFANEADIIRLGFKPGQKADIVSLWDDGRERR
VKGFTLLAFDIPAGQAAAYYPEVNPLVPLESTGDGSHTPTSKFVAIRLEAASENGLILAK
SA