Protein Info for Pf6N2E2_3828 in Pseudomonas fluorescens FW300-N2E2
Annotation: tRNA (cytidine(34)-2'-O)-methyltransferase (EC 2.1.1.207)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 65% identical to TRML_BURCH: tRNA (cytidine(34)-2'-O)-methyltransferase (trmL) from Burkholderia cenocepacia (strain HI2424)
KEGG orthology group: K03216, RNA methyltransferase, TrmH family, group 2 [EC: 2.1.1.-] (inferred from 96% identity to pfo:Pfl01_0337)MetaCyc: 60% identical to tRNA (cytidine/uridine-2'-O)-ribose methyltransferase (Escherichia coli K-12 substr. MG1655)
2.1.1.M27 [EC: 2.1.1.M27]; RXN-11860 [EC: 2.1.1.M27, 2.1.1.207]; 2.1.1.207 [EC: 2.1.1.M27, 2.1.1.207]
Predicted SEED Role
"tRNA (cytidine(34)-2'-O)-methyltransferase (EC 2.1.1.207)" (EC 2.1.1.207)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Anthocyanin biosynthesis
- Benzoxazinone biosynthesis
- Biosynthesis of alkaloids derived from shikimate pathway
- Carotenoid biosynthesis - General
- Flavonoid biosynthesis
- Histidine metabolism
- Insect hormone biosynthesis
- Naphthalene and anthracene degradation
- Phenylpropanoid biosynthesis
- Porphyrin and chlorophyll metabolism
- Tryptophan metabolism
- Tyrosine metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.1.1.-
Use Curated BLAST to search for 2.1.1.- or 2.1.1.207 or 2.1.1.M27
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160A039 at UniProt or InterPro
Protein Sequence (151 amino acids)
>Pf6N2E2_3828 tRNA (cytidine(34)-2'-O)-methyltransferase (EC 2.1.1.207) (Pseudomonas fluorescens FW300-N2E2) MFHVILFQPEIPPNTGNVIRLCANSGCHLHLIEPLGFEMDDKRLRRAGLDYHEYATLQRH ADLASCLESLGHPRLFAFTTKGSRPFHDARFVAGDAFLFGPESRGLPAEVLDALPADQRL RLPMREGCRSLNLSNTVAVAVYEAWRQNDFL