Protein Info for Pf6N2E2_3819 in Pseudomonas fluorescens FW300-N2E2

Annotation: Thiamine biosynthesis protein thiI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 484 TIGR00342: tRNA sulfurtransferase ThiI" amino acids 4 to 371 (368 residues), 330.3 bits, see alignment E=1.6e-102 PF02926: THUMP" amino acids 29 to 164 (136 residues), 62.2 bits, see alignment E=9.2e-21 PF02568: ThiI" amino acids 178 to 370 (193 residues), 202.9 bits, see alignment E=6.1e-64 TIGR04271: thiazole biosynthesis domain" amino acids 385 to 483 (99 residues), 117 bits, see alignment E=3.4e-38

Best Hits

Swiss-Prot: 90% identical to THII_PSEPF: tRNA sulfurtransferase (thiI) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K03151, thiamine biosynthesis protein ThiI (inferred from 100% identity to pba:PSEBR_a363)

MetaCyc: 49% identical to tRNA uridine 4-sulfurtransferase (Escherichia coli K-12 substr. MG1655)
tRNA sulfurtransferase. [EC: 2.8.1.4]; 2.8.1.- [EC: 2.8.1.4]

Predicted SEED Role

"Thiamine biosynthesis protein thiI"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A2F9 at UniProt or InterPro

Protein Sequence (484 amino acids)

>Pf6N2E2_3819 Thiamine biosynthesis protein thiI (Pseudomonas fluorescens FW300-N2E2)
MKLIVKVFPEITIKSRPVRMRFIRQLAKNIRAVLRDLDPAVVVNGVWDNLELETRVSEPK
VLKEMTERLSCMPGIAHFLQVDEYPLGDFDDILAKCMLHYGDALAGKIFSVRCKRAGKHP
FSSMDVEKYVGSQLRRQCGAAGISLKDPQIEVRIEIRDQRLFVIHSQHNSIGGYPLGSLE
QTLVLMSGGFDSTVAAYQIMRRGLMSHFCFFNLGGRAHELGVMEVAHFIWKKYGSSQRVL
FVSVPFEEVLGEILGKVDNSHMGVILKRMMLRAASRIADRLQIDALVTGEAISQVSSQTL
PNLSVIDCVTEKLVLRPLIASHKQDIIDQANEIGTAEFARHMPEYCGVISVNPKTAAKRG
RVEHEEKEFDMAVLERALENARLVPIDRVIDELGQDIQIEEVGEALAGQIIIDIRHPDDA
EDDPLEIAGIEVKTMPFYAVNARFKELDPTRQYLLYCDKGVMSRLHAHHLLSEGHANVRV
YRPS