Protein Info for Pf6N2E2_3799 in Pseudomonas fluorescens FW300-N2E2

Updated annotation (from data): Histidine ammonia-lyase (EC 4.3.1.3)
Rationale: Specifically important for utilizing L-Histidine. Automated validation from mutant phenotype: the predicted function (4.3.1.3) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: Histidine ammonia-lyase (EC 4.3.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01225: histidine ammonia-lyase
  • amino acids 7 to 508 (502 residues), 723.8 bits, E=4.7e-222
• PF00221: Lyase_aromatic
  • amino acids 11 to 470 (460 residues), 609 bits, E=2.9e-187

Best Hits

Swiss-Prot: 94% identical to HUTH_PSEPF: Histidine ammonia-lyase (hutH) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 100% identity to pba:PSEBR_a384)

MetaCyc: 84% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]

Predicted SEED Role

"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)

MetaCyc Pathways

• L-histidine degradation II (5/5 steps found)
• L-histidine degradation VI (7/8 steps found)
• L-histidine degradation I (4/4 steps found)
• L-histidine degradation III (4/6 steps found)

KEGG Metabolic Maps

• Histidine metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.3

Use Curated BLAST to search for 4.3.1.3

Sequence Analysis Tools

See A0A160A2E3 at UniProt or InterPro

Protein Sequence (510 amino acids)

>Pf6N2E2_3799 Histidine ammonia-lyase (EC 4.3.1.3) (Pseudomonas fluorescens FW300-N2E2)
MTALNLIPGQLSLAQLRDIYQQPVTLSLDASASAQIEASVACVEQILAENRTAYGINTGF
GLLASTRIASEDLENLQRSLVLSHAAGVGEPISDALVRLVMVLKVNSLSRGFSGIRRQVI
DALIALINAEVYPHIPLKGSVGASGDLAPLAHMSLVLLGEGKARYKGEWLPAVEALKVAG
LAPLTLAAKEGLALLNGTQVSTAFALRGLFEGEDLFAGALALGGLTVEAVLGSRSPFDAR
IHAARGQKGQIDAAAAYRDLLGERSEVSDSHQNCDKVQDPYSLRCQPQVMGACLTQFRQA
AEVLVIEANAVSDNPLVFAAEGDVISGGNFHAEPVAMAADNMALAIAEIGSLSERRISLM
MDKHMSQLPPFLVANGGVNSGFMIAQVTAAALASENKALSHPHSVDSLPTSANQEDHVSM
APAAGKRLWEMAENTRGILAVEWLAACQGLDLRGGLKTSTKLERARGLLRAQVPFYEKDR
FFAPDINAASELLASRCLNELVTAQLLPSL