Protein Info for Pf6N2E2_3744 in Pseudomonas fluorescens FW300-N2E2

Annotation: General secretion pathway protein F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 402 transmembrane" amino acids 167 to 190 (24 residues), see Phobius details amino acids 221 to 240 (20 residues), see Phobius details amino acids 373 to 396 (24 residues), see Phobius details TIGR02120: type II secretion system protein F" amino acids 4 to 401 (398 residues), 486.3 bits, see alignment E=4.5e-150 PF00482: T2SSF" amino acids 69 to 191 (123 residues), 103 bits, see alignment E=5.6e-34 amino acids 271 to 393 (123 residues), 86.9 bits, see alignment E=5.5e-29

Best Hits

Swiss-Prot: 63% identical to GSPF_PSEAE: Type II secretion system protein F (xcpS) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02455, general secretion pathway protein F (inferred from 63% identity to pap:PSPA7_1414)

Predicted SEED Role

"General secretion pathway protein F"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A1A5 at UniProt or InterPro

Protein Sequence (402 amino acids)

>Pf6N2E2_3744 General secretion pathway protein F (Pseudomonas fluorescens FW300-N2E2)
MPTFDYRAEDAQGRRCKGRVEADSPRHARQLLRERGLLPCELLAAKEPRTLGGWHAGTWL
SPADLALLTLQLSTLIQAGLPLEESLGAVAQQSQKRRVAHLLAAVRSRVMEGHALATALR
AFPQAFPELFCATVAAGEQSGHLGRVLEQLATYTQARQASRQRIQLALVYPLILMLACVA
IVAFLLGYVVPDVVKIFVDSGQPLPWLTQALIAMSDGLRRYGLLLLGALAGLLGLWRWSL
RQPAWRLRWHRLLLRLPLLGEVLRAMEAARFASTLAILSKSAVPLVEALHIAAQVIGNLT
IRARMADVARSVREGGTLTRGLELGGDIPPLMLHMIASGERAGELDSMLARAAEQQEASL
AARIALVVSLFEPAMLVLMGGVVLLIVMAILLPILSLNQLVN