Protein Info for Pf6N2E2_373 in Pseudomonas fluorescens FW300-N2E2
Annotation: Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 50% identical to GABT_ECOLI: 4-aminobutyrate aminotransferase GabT (gabT) from Escherichia coli (strain K12)
KEGG orthology group: None (inferred from 100% identity to pba:PSEBR_a3491)MetaCyc: 50% identical to 4-aminobutyrate aminotransferase GabT (Escherichia coli K-12 substr. MG1655)
5-aminovalerate transaminase. [EC: 2.6.1.48]; 4-aminobutyrate transaminase. [EC: 2.6.1.48, 2.6.1.19]; Acetylornithine transaminase. [EC: 2.6.1.48, 2.6.1.19, 2.6.1.11]
Predicted SEED Role
"Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)" (EC 2.6.1.19)
MetaCyc Pathways
- L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
- L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
- superpathway of arginine and polyamine biosynthesis (14/17 steps found)
- superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
- L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
- L-lysine degradation IV (5/5 steps found)
- L-ornithine biosynthesis I (5/5 steps found)
- superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
- L-lysine degradation X (5/6 steps found)
- 4-aminobutanoate degradation II (2/2 steps found)
- 4-aminobutanoate degradation III (2/2 steps found)
- superpathway of 4-aminobutanoate degradation (2/3 steps found)
- 4-aminobutanoate degradation I (1/2 steps found)
- GABA shunt II (2/4 steps found)
- L-lysine degradation I (4/7 steps found)
- GABA shunt I (1/4 steps found)
- L-lysine degradation III (2/6 steps found)
- 4-aminobutanoate degradation V (2/7 steps found)
- nicotine degradation I (pyridine pathway) (5/17 steps found)
- superpathway of L-lysine degradation (19/43 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Butanoate metabolism
- Glutamate metabolism
- Lysine biosynthesis
- Lysine degradation
- Propanoate metabolism
- Urea cycle and metabolism of amino groups
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.6.1.11, 2.6.1.19, 2.6.1.48
Use Curated BLAST to search for 2.6.1.11 or 2.6.1.19 or 2.6.1.48
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A165YTD0 at UniProt or InterPro
Protein Sequence (430 amino acids)
>Pf6N2E2_373 Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19) (Pseudomonas fluorescens FW300-N2E2) MNSKVEETPSLLRQRDQFVPRGLVTAHPLVIDRAQGSELWDVDGKRYLDFVGGIGVLNIG HNHPKVVAAVQAQLQKISHACFQVVAYKPYLDLAQRLCEMIGGQHAYKAAFFTSGAEAVE NAVKIARAHTNRSAVIAFRGGFHGRTLLGTTLTGMSQPYKQNFGPFAPEVFHTPYPNAYR GVTSAMALQALDELLATQVAPERVAAIIIEPVQGDGGFLSAPAEFLQGLRALTQKHGIVL ILDEIQTGFGRTGKWFGFQHAGIQPDLVTVAKSLAGGLPLSGVVGKAEIMDAPLPGGLGG TYGGNALSCAAALAVIEAYEQEQLLARGEVLGERLRQGLLHLQTRYPQIGDVRGSGFMLA IELIKHDEARTPDADLNQRLIDEARAGGLLVIKCGVYRNVLRFLAPLVTSEAQIDEALQI LDGALARVLG