Protein Info for Pf6N2E2_3686 in Pseudomonas fluorescens FW300-N2E2

Annotation: Acyl-CoA dehydrogenase (EC 1.3.8.7)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 transmembrane" amino acids 297 to 317 (21 residues), see Phobius details PF12418: AcylCoA_DH_N" amino acids 9 to 34 (26 residues), 25.3 bits, see alignment (E = 3.3e-09) PF02771: Acyl-CoA_dh_N" amino acids 40 to 156 (117 residues), 42.1 bits, see alignment E=2.6e-14 PF02770: Acyl-CoA_dh_M" amino acids 162 to 269 (108 residues), 43 bits, see alignment E=1e-14 PF00441: Acyl-CoA_dh_1" amino acids 285 to 451 (167 residues), 61 bits, see alignment E=4e-20 PF12806: Acyl-CoA_dh_C" amino acids 471 to 595 (125 residues), 104.5 bits, see alignment E=1.1e-33

Best Hits

KEGG orthology group: K00248, butyryl-CoA dehydrogenase [EC: 1.3.8.1] (inferred from 98% identity to pba:PSEBR_a472)

Predicted SEED Role

"Acyl-CoA dehydrogenase (EC 1.3.8.7)" (EC 1.3.8.7)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7

Use Curated BLAST to search for 1.3.8.1 or 1.3.8.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161GSW1 at UniProt or InterPro

Protein Sequence (600 amino acids)

>Pf6N2E2_3686 Acyl-CoA dehydrogenase (EC 1.3.8.7) (Pseudomonas fluorescens FW300-N2E2)
MSETLLSSRNLAFELYEVLDAEGLTQRERFAEHNRETFDAALATARNIAEKFFAPHNRKN
DENEPRYENGEAILIPEVKPAVDAFLEAGFLNAARSFEAGGMQLPTLLSQACFAHFQSAN
AASTSYPFLTMGAANLIESFGSEEQKRRFLQPMIDGRFFGTMALTEPHAGSSLADIRTRA
EPASDGTYRLKGNKIFISGGDHPLSENIVHMVLAKLPDAPPGVKGISLFIVPKFLVNDDG
SLGQRNDVLLAGLFHKMGWRGTTSTALNFGDNGACVGYLVGKPHHGLSYMFQMMNEARIG
VGMGAVMLGYAGYLYSLEYARERPQGRVPDSKDPTTAPVTIIQHADVRRMLLTQKAYVEG
SFDLGLYAARLFDDTTTLESEAERRRAHELLDLLTPIVKSWPSEFCLKANELAIQILGGH
GYTREYPVEQYYRDNRLNPIHEGTHGIQSLDLLGRKLAQNGGAGLKQLVRLVADTTERAQ
AHPSLTPLRQPLEALVARLQTVTLGLLTDLAQGKVNSSLANSALYLKVFGHMVIGWRWLE
QAIRAEEGLAKGDTADSDFYKGKLQAARYFLTWEVPGCHHELAILEARDDTCLGMRDEWF