Protein Info for Pf6N2E2_368 in Pseudomonas fluorescens FW300-N2E2

Annotation: Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 22 to 479 (458 residues), 557.8 bits, E=8.7e-172
• TIGR01780: succinate-semialdehyde dehydrogenase
  • amino acids 33 to 478 (446 residues), 680.7 bits, E=4.8e-209

Best Hits

Swiss-Prot: 61% identical to SLAD_PSEPU: 3-sulfolactaldehyde dehydrogenase (PpSQ1_00395) from Pseudomonas putida

KEGG orthology group: K00135, succinate-semialdehyde dehydrogenase (NADP+) [EC: 1.2.1.16] (inferred from 99% identity to pba:PSEBR_a3496)

MetaCyc: 61% identical to succinate-semialdehyde dehydrogenase (NADP+) GabD (Escherichia coli K-12 substr. MG1655)
SUCCSEMIALDDEHYDROG-RXN [EC: 1.2.1.79]; Glutarate-semialdehyde dehydrogenase. [EC: 1.2.1.79, 1.2.1.20]

Predicted SEED Role

No annotation

MetaCyc Pathways

• superpathway of L-arginine and L-ornithine degradation (11/13 steps found)
• L-lysine degradation IV (5/5 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (9/11 steps found)
• L-lysine degradation X (5/6 steps found)
• 4-aminobutanoate degradation II (2/2 steps found)
• 4-aminobutanoate degradation III (2/2 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• 4-hydroxyphenylacetate degradation (6/8 steps found)
• superpathway of 4-aminobutanoate degradation (2/3 steps found)
• L-lysine degradation I (4/7 steps found)
• L-lysine degradation III (2/6 steps found)
• nicotine degradation II (pyrrolidine pathway) (3/11 steps found)
• nicotine degradation I (pyridine pathway) (5/17 steps found)
• superpathway of L-lysine degradation (19/43 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Glutamate metabolism
• Lysine degradation
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.16

Use Curated BLAST to search for 1.2.1.16 or 1.2.1.20 or 1.2.1.79

Sequence Analysis Tools

See A0A165YT81 at UniProt or InterPro

Protein Sequence (485 amino acids)

>Pf6N2E2_368 Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16) (Pseudomonas fluorescens FW300-N2E2)
MLKNRLKDPSLLAELAYVDGQWIGADNAATLDVIDPATGHSLARVPAMQGVETRRAIEAA
ERAWPAWRARPAAERAALLERWYQAMMDNLDDLALIMTCEQGKPLSEAKGEIRYGAGFAK
WFAEEARRVYGETIPAPSGDRRLLTLKQPVGVCAAITPWNFPNAMITRKCAPALAAGCPV
IVKPSDLTPLSALALAVLAERVGIPAGVFNVVTGMPAGIGEELTGNPTVRKISFTGSTAV
GRLLMRQSAEHIKRLSLELGGNAPFIVFDDADLEQAVAGIMLSKFRNAGQTCVCANRILV
QDGIYERFAARLVEEVGKLKVGNGLEAGVMIGPLINLAAVNKVARHIDDALSQGARLLCG
GVPEGDSQFVQPTVLGEAHAGMLLANEETFGPVAPLMRFTTEEQALALANATPYGLGAYY
FTQDLRRSWRFGEALEFGMVGLNTGIISMEVAPFGGIKQSGLGREGSKYGLDEYLEVKAF
HIGGL