Protein Info for Pf6N2E2_3672 in Pseudomonas fluorescens FW300-N2E2

Annotation: Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 983 PF03710: GlnE" amino acids 50 to 298 (249 residues), 286.5 bits, see alignment E=1.7e-89 amino acids 578 to 831 (254 residues), 309.3 bits, see alignment E=2e-96 PF08335: GlnD_UR_UTase" amino acids 319 to 457 (139 residues), 108 bits, see alignment E=4.1e-35 amino acids 877 to 951 (75 residues), 31.8 bits, see alignment 1.3e-11

Best Hits

Swiss-Prot: 82% identical to GLNE_PSESM: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 99% identity to pba:PSEBR_a484)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A004 at UniProt or InterPro

Protein Sequence (983 amino acids)

>Pf6N2E2_3672 Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42) (Pseudomonas fluorescens FW300-N2E2)
MSLPSLVPISETVPAILQSLVSRAEQSFRAVVALLDDDHGLSAWTPQRWEAFNRIAAASD
FVIEQSLRDPVMLLELVRSGELDRSLAPGEMCAQIGAAVRAAETEEELGRVLRRQRTRQQ
VRIIWRDLTRQADLVQTCRDLSDMADTCIDQAYRWLYQRLSQQFGTPTGRRSGEPQQMVI
LGMGKLGAVELNLSSDIDLIFAYPEGGETVGVKRPLDNQEFFIRVGQRLIKALDPMTVDG
FVFRVDMRLRPYGSAGALVLSFNALEQYYQDQGRDWERYAMIKARVVAGDQVAGAQLLDM
LRPFVYRRYLDFSAIEALRTMKQLIQQEVRRKGMADNIKLGSGGIREVEFIAQAFQLIHG
GRDLSLQQRPLLKVLGTLEGQGYLPAKVIDELRQGYEFLRYTEHAIQAIADRQTQMLPDN
PQDQARIAFMLGFADWSAFHEQLMYWRGRVAWHFGQVIADPDEEAGGESEVVVGGEWLPL
WEDSQDEEAACRQLEEGGFVDAPKALKALAALRGSPQLRAMQRLGRERLDAFIPRLLAQA
VEHADPDLVLERVLPLVEAVARRSAYLVLLTENPGALRRLLTLCAASPWIAEQITRFPLL
LDELLNEGRLFKPPLAPELAAELRERLTRIPEDDLEQQMEALRHFKLAHRLRVAASEIAG
SLPLMKVSDYLTWLAEAILEQVLALAWRQTAAKHGVPLRTDGSLCDPGFIIVGYGKVGGL
ELGHGSDLDLVFIHDGDPHAETDGPKPIDGAQFFTRLGQRIIHLLTAQTNSGQLYEVDMR
LRPSGASGLLVSSLGAFARYQENEAWTWEHQALVRARVLVGSQDVGQAFEKVRGQVLGRK
RDLPTLRQEVSEMRAKMRDNLGSKVTAAGTAPNAFEATAPFDLKQDAGGIVDIEFMVQYA
ALAWSEEHPSLVRYTDNIRILDGLQEVGLMSAEDATLLREAYKAYRSAAHRQALQKDAGV
IAGDQFVEERRQVLRIWRELGLS