Protein Info for Pf6N2E2_3617 in Pseudomonas fluorescens FW300-N2E2

Annotation: N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 475 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF11741: AMIN" amino acids 35 to 133 (99 residues), 74.4 bits, see alignment E=1e-24 PF01520: Amidase_3" amino acids 178 to 396 (219 residues), 151 bits, see alignment E=5.4e-48 PF01476: LysM" amino acids 425 to 467 (43 residues), 50.9 bits, see alignment 1.8e-17

Best Hits

KEGG orthology group: K01448, N-acetylmuramoyl-L-alanine amidase [EC: 3.5.1.28] (inferred from 99% identity to pba:PSEBR_a538)

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A211 at UniProt or InterPro

Protein Sequence (475 amino acids)

>Pf6N2E2_3617 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) (Pseudomonas fluorescens FW300-N2E2)
MMGFGMRFRAVVAVVGLLLTALAVDAVAETKVNSVRLWRAPDNTRLVFDLTGPVQHSVFT
LTAPDRLVIDINGASLGAPLNVATANTPITAMRSAQRTPTDLRVVIDLKKAVTPKSFTLA
PNAQYGNRLVVDLFDNPADATPPPPPTNVATVPAVPVTPTEPALKLPPAPAGKRDIIVVI
DAGHGGEDPGASGSRGQREKDVVLSIARELQRQVNGMKGFRAELTRTGDYFIPLRGRTEI
ARKKGADLFVSIHADAAPSAAAFGASVFALSDRGATSETARWLADSENRSDLIGGAGNVS
LDDKDRMLAGVLLDLSMTASLTSSLNVGQKVLSNIGRVTPLHKQRVEQAGFMVLKSPDIP
SILVETGFISNANEASKLSSANHQQALARSISSGVRQFFQQNPPPGTYIAWLRDSGKIAQ
GPRDHRVSPGETLAMIAVRYQVSPATLRSANNLKSDELKIGQTLTIPGNEVAVQR