Protein Info for Pf6N2E2_3613 in Pseudomonas fluorescens FW300-N2E2

Annotation: GTP-binding protein HflX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03156: GTP-binding protein HflX
  • amino acids 11 to 361 (351 residues), 509 bits, E=3e-157
• PF13167: GTP-bdg_N
  • amino acids 28 to 113 (86 residues), 105.3 bits, E=4.2e-34
• PF16360: GTP-bdg_M
  • amino acids 115 to 193 (79 residues), 100.5 bits, E=1.3e-32
• PF02421: FeoB_N
  • amino acids 200 to 323 (124 residues), 27.5 bits, E=4.1e-10
• PF01926: MMR_HSR1
  • amino acids 201 to 319 (119 residues), 72.4 bits, E=6.8e-24

Best Hits

Swiss-Prot: 60% identical to HFLX_ECOLI: GTPase HflX (hflX) from Escherichia coli (strain K12)

KEGG orthology group: K03665, GTP-binding protein HflX (inferred from 100% identity to pba:PSEBR_a542)

MetaCyc: 60% identical to ribosome rescue factor HflX (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein HflX" in subsystem Hfl operon or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A0A160A241 at UniProt or InterPro

Protein Sequence (433 amino acids)

>Pf6N2E2_3613 GTP-binding protein HflX (Pseudomonas fluorescens FW300-N2E2)
LFFERHGGGERAILVHLDGQDPEAREDPQEFQELALSAGAETVAFFNVPRHRPTAKFLIG
SGKVEELRDLVKAEQVDLVIFNHILTPSQERNLERVFECRVIDRTGLILDIFAQRARTHE
GKLQVELAQLEHMSTRLVRGWTHLERQKGGIGLRGPGETQLETDRRLLRVRLRQIKGRLE
KVRSQREQSRRGRKRADIPTVSLVGYTNAGKSTLFNNVTQSDVYAADQLFATLDPTLRRL
DLDDLGPIVLADTVGFIRHLPHKLVEAFRATLEESSNSDLLLHVIDAAEPDRMLQIEQVM
VVLGEIGAQDLPILEVYNKLDLLEGVEPQIQRDADGKPQRVWLSARDGTGLDLLKQAVAE
LLGNDLFVGTLKLPQRFARLRAQFFELGAVQKEEHDDEGVCLLAVRLPRAELNRLVSREG
LQPMEFIEQHTLQ