Protein Info for Pf6N2E2_3574 in Pseudomonas fluorescens FW300-N2E2
Annotation: Mannose-6-phosphate isomerase (EC 5.3.1.8)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01809, mannose-6-phosphate isomerase [EC: 5.3.1.8] (inferred from 97% identity to pba:PSEBR_a576)Predicted SEED Role
"Mannose-6-phosphate isomerase (EC 5.3.1.8)" in subsystem Alginate metabolism or Mannose Metabolism (EC 5.3.1.8)
MetaCyc Pathways
- GDP-mannose biosynthesis (4/4 steps found)
- colanic acid building blocks biosynthesis (9/11 steps found)
- β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
- D-mannose degradation I (1/2 steps found)
- D-mannose degradation II (1/2 steps found)
- mannitol degradation II (2/4 steps found)
- mannitol biosynthesis (1/3 steps found)
- 1,5-anhydrofructose degradation (2/5 steps found)
- β-(1,4)-mannan degradation (2/7 steps found)
- superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (7/14 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 5.3.1.8
Use Curated BLAST to search for 5.3.1.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A159ZY35 at UniProt or InterPro
Protein Sequence (377 amino acids)
>Pf6N2E2_3574 Mannose-6-phosphate isomerase (EC 5.3.1.8) (Pseudomonas fluorescens FW300-N2E2) MPDASRPAPQPELTALFATVRQHFHDVIVPMWQGPGWNAELALPYESLDAEHVPLPPQRY RAMACARQLYVFANLIGEVPQAEERASALFRSLQRHFHDAEHGGWFYSIDPQGAPLDTGK DLYTHAFILFACAHYWGKVREPLVESVLNASLEVIARRFASADGLYEASLKRDWSTTGSG PLQNPLMHLAEAFLATLSVREDANVRQALLSLCDGMSKHFIEPQQRVMMEKPLKAVDNWF EPGHQFEWYFLLASSPLLRDGKLHAALEHSFGHTEQRGVAPDSGAVCATLALGSQAGPRD ATQRIWAQAEYLRALTLRPGSQGLLLRQLRALQQHFLHAKGWNECLDAAGVVSRRDMPST TPYHLLTCYRGLADYLA