Protein Info for Pf6N2E2_3558 in Pseudomonas fluorescens FW300-N2E2

Annotation: DnaJ-class molecular chaperone CbpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 313 PF00226: DnaJ" amino acids 5 to 66 (62 residues), 83.7 bits, see alignment E=7.9e-28 PF01556: DnaJ_C" amino acids 130 to 287 (158 residues), 143.4 bits, see alignment E=5.8e-46

Best Hits

Swiss-Prot: 73% identical to CBPA_PSEPW: Curved DNA-binding protein (cbpA) from Pseudomonas putida (strain W619)

KEGG orthology group: K05516, curved DNA-binding protein (inferred from 98% identity to pba:PSEBR_a591)

Predicted SEED Role

"DnaJ-class molecular chaperone CbpA" in subsystem Protein chaperones

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZZX1 at UniProt or InterPro

Protein Sequence (313 amino acids)

>Pf6N2E2_3558 DnaJ-class molecular chaperone CbpA (Pseudomonas fluorescens FW300-N2E2)
MDFKDYYKILGVEPTADDATIKAAYRKLARKYHPDVSKEKDAETKFKDVSEAYEALKSAD
KRAEYDDLRRYGQHGQPFQGPPGWQSRGGFGGGQDTGDFSDFFSSIFGNRGPGFGGGQSG
RSAGRRGQDVEMELPIFLEETLSSESKKVSFQVPQYNAAGQHVSNTSKSLNVKIPAGVTD
GERIRLKGQGAPGIGGGANGDLYLTIRFAPHPKFDVEGQDLIITLPLAPWELALGTEVAV
PTLTGKINLKVPAGSQNGQRMRAKGHGLRNKAGERGYLFVQLKAVMPKANDEAVKALWAE
LAKKAAFDPRENF