Protein Info for Pf6N2E2_352 in Pseudomonas fluorescens FW300-N2E2

Annotation: Opine oxidase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01266: DAO
  • amino acids 7 to 355 (349 residues), 243.6 bits, E=1.4e-75
• PF13450: NAD_binding_8
  • amino acids 10 to 37 (28 residues), 26.5 bits, see alignment (E = 1.9e-09)

Best Hits

KEGG orthology group: K00303, sarcosine oxidase, subunit beta [EC: 1.5.3.1] (inferred from 98% identity to pba:PSEBR_a3510)

Predicted SEED Role

"Opine oxidase subunit B"

MetaCyc Pathways

• glycine betaine degradation I (6/8 steps found)
• creatinine degradation I (1/4 steps found)
• creatinine degradation II (1/5 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1

Sequence Analysis Tools

See A0A165YSQ9 at UniProt or InterPro

Protein Sequence (393 amino acids)

>Pf6N2E2_352 Opine oxidase subunit B (Pseudomonas fluorescens FW300-N2E2)
MKPEKSDVLIIGGGIMGSASAFFLRQRGHSVTLLERDLIGQYASGVNFGNVRRQGRFLGQ
LELSNRSYGLWKRLPELIGDDLEFIPSGHMRVCYREDEIAELEAYAAAPEARELDLQIIT
GKALHARFPFLGAEVKGGSYAPHDGHANPRLAAPAFARAATRAGASIRERTEVATVRKEN
DHFHVTTTNGQLFIAEQLLITAGAWGAKLSEQFDEAVPLQANGPQMSVTEPVPYALPTVI
GVFTKIKEEVIYFRQIPRGNLIIGGGNRCKPDMINRRAYFKPESLLNQMQQMSRLLPGVA
NLNIIRVWSGIESYTPDSLPIMGPSGKVKGLFYAFGFCGHGFQLGPGVGDVMAELISTGS
TRTLISPFDIRRFTEPTALTMPQMSTPGTGRLI