Protein Info for Pf6N2E2_3517 in Pseudomonas fluorescens FW300-N2E2

Annotation: tRNA dihydrouridine synthase B (EC 1.-.-.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 335 TIGR00737: putative TIM-barrel protein, nifR3 family" amino acids 6 to 320 (315 residues), 417.4 bits, see alignment E=1.9e-129 PF01207: Dus" amino acids 17 to 320 (304 residues), 352.6 bits, see alignment E=9.5e-110

Best Hits

Swiss-Prot: 88% identical to DUSB_PSESM: tRNA-dihydrouridine synthase B (dusB) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a629)

MetaCyc: 60% identical to tRNA-dihydrouridine synthase B (Escherichia coli K-12 substr. MG1655)
1.1.1.-

Predicted SEED Role

"tRNA dihydrouridine synthase B (EC 1.-.-.-)" (EC 1.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZZN6 at UniProt or InterPro

Protein Sequence (335 amino acids)

>Pf6N2E2_3517 tRNA dihydrouridine synthase B (EC 1.-.-.-) (Pseudomonas fluorescens FW300-N2E2)
MSAVRIGPYTLQNGLVLAPMAGVTDQPFRQLCKRLGAGLVVSEMVTSDMSLWNTRKSRLR
MIHEGDPEPRSVQIAGGDAQMLAEAARANVELGAQIIDINMGCPAKKVCNKAAGSALLKD
EALVTEILQAVVAAVDVPVTLKIRTGWDRENKNGLVVAKIAEQAGITALAVHGRTRADLY
TGDAEYDTIAAIKQAVSIPVFANGDIDSPEKARYVLDATGADGLLIGRAAQGRPWIFREI
EHFLRTGEKLAAPQLSEVERILLEHLAALHAFYGEVMGVRIARKHVGWYLATLPGAREFR
ARFNRLDGTQAQCADVQAFFAERYKSLTGDEGVAA