Protein Info for Pf6N2E2_3384 in Pseudomonas fluorescens FW300-N2E2

Annotation: Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 223 transmembrane" amino acids 126 to 143 (18 residues), see Phobius details PF02589: LUD_dom" amino acids 44 to 220 (177 residues), 114.2 bits, see alignment E=3.3e-37

Best Hits

KEGG orthology group: K00782, hypothetical protein (inferred from 97% identity to pba:PSEBR_a751)

Predicted SEED Role

"Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG" in subsystem Lactate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165ZFA7 at UniProt or InterPro

Protein Sequence (223 amino acids)

>Pf6N2E2_3384 Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG (Pseudomonas fluorescens FW300-N2E2)
MSAKENILAKLRKSLTGATPVADNFDVELVTQTYRYAPEERIPQLRKLMEAVHTEIHLTS
DEGWPTLLAQLLRDRQLPSLLIAPTTAHGQKITQFWANNPDLPTLKAYDRPIEEWKAELF
NDTPASLTGTLGAIAATGSLILWPTREEPRLMSLVPPVHFALLKASEIRDNFYEVQQEFE
WAQGMPTNALLVSGPSKTADIEQVLAYGAHGPKDLVVLILEDQ