Protein Info for Pf6N2E2_3368 in Pseudomonas fluorescens FW300-N2E2

Annotation: Chaperone protein DnaK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 638 TIGR02350: chaperone protein DnaK" amino acids 3 to 603 (601 residues), 946.4 bits, see alignment E=3e-289 PF00012: HSP70" amino acids 4 to 603 (600 residues), 871.3 bits, see alignment E=4.6e-266 PF06723: MreB_Mbl" amino acids 115 to 377 (263 residues), 42.2 bits, see alignment E=7.3e-15

Best Hits

Swiss-Prot: 96% identical to DNAK_PSEF5: Chaperone protein DnaK (dnaK) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K04043, molecular chaperone DnaK (inferred from 100% identity to pba:PSEBR_a764)

MetaCyc: 75% identical to chaperone protein DnaK (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein DnaK" in subsystem Heat shock dnaK gene cluster extended or Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0N9W600 at UniProt or InterPro

Protein Sequence (638 amino acids)

>Pf6N2E2_3368 Chaperone protein DnaK (Pseudomonas fluorescens FW300-N2E2)
MGKIIGIDLGTTNSCVSVLENGKAKVIENAEGARTTPSIIAYANDGEILVGQSAKRQAVT
NPHNTLYAVKRLIGRKFDEEVVQKDIKMVPYKIAKADNGDAWVEVNGQKMAPPQISAEIL
KKMKKTAEDYLGEAVTEAVITVPAYFNDSQRQATKDAGRIAGLDVKRIINEPTAAALAYG
MDKAKGDHTVIVYDLGGGTFDVSVIEIAEVDGEHQFEVLATNGDTFLGGEDFDIRLIDYL
VDEFKKESGMNLKGDPLAMQRLKEAAEKAKIELSSSQSTDVNLPYITADATGPKHLNVKI
SRAKLEALVEDLVQRTIEPCRIAMKDAGIDVGSINDVILVGGQTRMPLVQKLVTDFFGKE
ARKDVNPDEAVAMGAAIQGAVLAGDVKDVLLLDVSPLTLGIETMGGVMTALIEKNTTIPT
KKSQVFSTADDNQGAVTIHVLQGERKQAAQNKSLGKFDLAEIPPAPRGVPQIEVTFDIDA
NGILHVGAKDKATGKTQSIVIKANSGLSEEEIQQMVRDAEVNAEEDRKFEELASARNQGD
ALVHSTRKMIADAGEKVTAEEKTAIEAAVVALEAAVKGDDKAAIEAKIEELSKVSAPVAQ
KMYAEQAQPAEGAAAQGESAEKADDVVDAEFEEVKDHK