Protein Info for Pf6N2E2_3343 in Pseudomonas fluorescens FW300-N2E2

Annotation: AmpE protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 278 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 45 to 65 (21 residues), see Phobius details amino acids 71 to 89 (19 residues), see Phobius details amino acids 145 to 167 (23 residues), see Phobius details amino acids 193 to 214 (22 residues), see Phobius details amino acids 259 to 277 (19 residues), see Phobius details PF17113: AmpE" amino acids 21 to 207 (187 residues), 52 bits, see alignment E=3.4e-18

Best Hits

KEGG orthology group: K03807, AmpE protein (inferred from 99% identity to pba:PSEBR_a787)

Predicted SEED Role

"AmpE protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZZ38 at UniProt or InterPro

Protein Sequence (278 amino acids)

>Pf6N2E2_3343 AmpE protein (Pseudomonas fluorescens FW300-N2E2)
MSFLVLLLAVWIEKFSALRQRVQRDGGWLHELNKLEASPRWVNRPWLVLTVMVLLPVALL
ALLLWVLEPVAYGLLALPVHLLVVIYSLGRGDLLAGLGPFRDAWRREDLQAAAHVAKRDL
DIEANDGEQLLERVQGHLLWQAYQSFFAVIFWYFVLGPVAALSYRLLALAAEHSQNPGVA
ERAAQMRHAFDWVPVRLLAASFALVGNFVAVSRVMLHELLNWNISAADLIDKVGLVAGEI
PRPVAGPDGINSLDRLWELLLRAAVLWYAGFALWTVLA