Protein Info for Pf6N2E2_3339 in Pseudomonas fluorescens FW300-N2E2

Annotation: Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 954 transmembrane" amino acids 589 to 612 (24 residues), see Phobius details PF00359: PTS_EIIA_2" amino acids 5 to 140 (136 residues), 101.3 bits, see alignment E=1.2e-32 PF00381: PTS-HPr" amino acids 286 to 366 (81 residues), 62.5 bits, see alignment E=8.2e-21 TIGR01003: phosphocarrier, HPr family" amino acids 286 to 365 (80 residues), 48.3 bits, see alignment E=7.9e-17 PF05524: PEP-utilisers_N" amino acids 405 to 520 (116 residues), 76.7 bits, see alignment E=4.5e-25 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 407 to 952 (546 residues), 496.4 bits, see alignment E=1e-152 PF00391: PEP-utilizers" amino acids 545 to 617 (73 residues), 76.1 bits, see alignment 3.5e-25 PF02896: PEP-utilizers_C" amino acids 646 to 926 (281 residues), 363.2 bits, see alignment E=2.4e-112

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 99% identity to pba:PSEBR_a791)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZXH0 at UniProt or InterPro

Protein Sequence (954 amino acids)

>Pf6N2E2_3339 Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9) (Pseudomonas fluorescens FW300-N2E2)
MLELTLEQISMAQTAVDKDAALQLLADKLVADGLVAEGYLAGLQAREAQGSTFLGQGIAI
PHGTPQTRDLVHSTGVRLLQFPEGVDWGDGQIVYLAIGIAAKSDEHLRLLQLLTRALGET
DLGQALRRAGSAEALLKLLQGAPQELALDAQMIGLGVSADDFEELVWRGARLLRQADCVS
NGFAGVLQQVDALPLGDGLWWLHSEQTVKRPGLAFVTPDKPIRYLGQPLSGLFCLASLGE
AHQALLERLCALLIEGRGHELGRATSSRKVLEVLGGELPADWPSARIGLANAHGLHARPA
KILAQLAKSFEGEIRVRIVDGQDSAVSAKSLSKLLSLGARRGQVLEFIAEPSIANDALPA
LLAAIEEGLGEEVEPLPPPSAPRETVMAEVATVMLAPESGSLIQAVAAAPGIAIGPAHIQ
VLQAIDYPLRGESAAIERERLQNALNQVRRDIQGLIERAKAKAIREIFITHQEMLDDPEL
SDEVDTRLKLGESAQAAWMGVVEAAAKEQEALQDALLAERAADLRDVGRRVLAQLCGVET
PNEPDQPYILVMDEVGPSDVARLDPTRVAGILTARGGATAHSAIVARALGIPALVGAGAA
VLLLAPGTSLLLDGQRGRLHVDPDAATLQRAKEERDTREQRLKVAAEQRHEPALTRDGHA
VEVFANIGESAGVAGAVEQGAEGIGLLRTELIFMAHSQAPDEATQEAEYRKVLDGLAGRP
LVVRTLDVGGDKPLPYWPIAKEENPFLGVRGIRLTLQRPQVMEAQLRALLRSADNRPLRI
MFPMVGSVDEWRQARAMTERLRQEIPVADLQLGIMIEVPSAALLAPVLAKEVDFFSVGTN
DLTQYTLAIDRGHPTLSAQADGLHPAVLQLIDITVRAAHAHGKWVGVCGELAADPLAVPV
LVGLGVDELSVSARSIAEVKARVRELSLAQVQTLAQEALAVGSADDVRALVEAL