Protein Info for Pf6N2E2_3335 in Pseudomonas fluorescens FW300-N2E2
Annotation: Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00380, sulfite reductase (NADPH) flavoprotein alpha-component [EC: 1.8.1.2] (inferred from 97% identity to pba:PSEBR_a795)Predicted SEED Role
"Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB"
MetaCyc Pathways
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
- superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
- assimilatory sulfate reduction I (4/4 steps found)
- assimilatory sulfate reduction III (3/3 steps found)
- superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.8.1.2
Use Curated BLAST to search for 1.8.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160A1E3 at UniProt or InterPro
Protein Sequence (847 amino acids)
>Pf6N2E2_3335 Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB (Pseudomonas fluorescens FW300-N2E2) MNKASDVLKKTLFQLHWFFGISAGLVLALMGVTGAVVSFQDEILRALNPQVLMVEKQPAG VLPPAELVEQIEAASGKTVAMLWVETDSGNAARVIFAAPPGEKRGAMRYFNPYNGQFMGD VTGQDFFGLMLRLHRVLTLDDVGRQITGACTLILVFFCLSGLYLRWPRQWKSWRAWLTLD WGKKGRSFNWDLHSVAGTWCLAFYLLAALTGLTWSYDWYNKGVTRLLSDSPKEERVRNRG PAPSGPLPVADYRAMWSSIYSAAGPGLSTYNVRMPPVAGQPATVFYLLKNSPHDRALNQL ILDPTTGVISRHSRYSDKSLKAQLLTSVYALHIGSYFGLVGRILVTVAALAMPLFFITGW LLYLDRRRKQRQARNARQALSANPTDAPAWLIGFASQSGFAERLAWQTAGQLQAAGLPVK VQPLAGVREQDLNDATHALFVVSTFGDGEGPDSARGFERKVLGQALSLERLQYSVLGLGD RQYEHFCGFARRLHAWLAEHGGKTLFAPVEVDSGDPYALRHWQQQLAELTGQAPLDTWQA PSFENWTLSQRTLLNPDSSGSGVYLLGFTAPGPSSWLAGDLVEILPRNDLSAIEHFLDGL GLSGHARVQIDGLSQSLDQALATRQLPENRAHLVGLHAQALVDALAPLAIREYSIASIPA DGVLELIVRQERHADGRLGVGSGWLTEHAPLGSAVSLRLRRNSSFHLPPQGVPMILLGNG TGLAGLRSLLKARVTEGIQRNWLLFGERNREHDFHCRDELQEWVTSGDLERLDLAFSRDQ KQKVYVQDRLLESAALLKQWVADGAAIYVCGSLQGMASGVDHVLNNVLGREEVERLIEQG RYRRDVY