Protein Info for Pf6N2E2_3278 in Pseudomonas fluorescens FW300-N2E2

Annotation: TldE protein, part of TldE/TldD proteolytic complex

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 452 PF01523: PmbA_TldD_1st" amino acids 38 to 102 (65 residues), 50.5 bits, see alignment E=2.9e-17 PF19290: PmbA_TldD_2nd" amino acids 130 to 236 (107 residues), 78.2 bits, see alignment E=9.3e-26 PF19289: PmbA_TldD_3rd" amino acids 243 to 451 (209 residues), 233.2 bits, see alignment E=3.3e-73

Best Hits

Swiss-Prot: 49% identical to PMBA_HAEIN: Metalloprotease PmbA homolog (pmbA) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K03592, PmbA protein (inferred from 99% identity to pba:PSEBR_a836)

Predicted SEED Role

"TldE protein, part of TldE/TldD proteolytic complex"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165ZEP1 at UniProt or InterPro

Protein Sequence (452 amino acids)

>Pf6N2E2_3278 TldE protein, part of TldE/TldD proteolytic complex (Pseudomonas fluorescens FW300-N2E2)
VENTMSAVQSVGPQALPALQEQVEQILAEAKRQGASACEVAVSLEQGLSTSVRQREVETV
EFNRDQGFGITLYVGQRKGSASTSASGPEAIRETVAAALAIAKHTSEDEASGLADAALMC
KELRDFDLFHAWDITPEQAIEQALRCEAAAFEADSRIKNADGTTLNTHQGCRVYGNSHGF
IGGYASTRHSLSCVMIAEADGQMQRDYWYDVNRQGTLLADPVSIGRKAAQRAASRLGARP
VPTCEVPVLFSAELAGGLFGSFLSAVSGGNLYRKSSFLEGALGQKLFPEWMTIDERPHLM
QAMGSSAFDGDGLATYAKPFVEKGELVSYILGTYSGRKLGMPSTANAGGVHNLFVTHGEE
DQAALLRRMGRGLLVTELMGHGLNMVTGDYSRGAAGFWVENGEIQFPVQEVTIAGNMRDM
FKQIVAVGNDLELRSNIRTGSVLIERMTVAGS