Protein Info for Pf6N2E2_3258 in Pseudomonas fluorescens FW300-N2E2

Annotation: Uncharacterized ABC transporter, periplasmic component YrbD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 155 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details TIGR04430: outer membrane lipid asymmetry maintenance protein MlaD" amino acids 2 to 147 (146 residues), 180.8 bits, see alignment E=6.8e-58 PF02470: MlaD" amino acids 39 to 116 (78 residues), 74.2 bits, see alignment E=4.2e-25

Best Hits

Swiss-Prot: 46% identical to MLAD_ECOLI: Intermembrane phospholipid transport system binding protein MlaD (mlaD) from Escherichia coli (strain K12)

KEGG orthology group: K02067, putative ABC transport system substrate-binding protein (inferred from 99% identity to pba:PSEBR_a854)

Predicted SEED Role

"Uncharacterized ABC transporter, periplasmic component YrbD"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZYW8 at UniProt or InterPro

Protein Sequence (155 amino acids)

>Pf6N2E2_3258 Uncharacterized ABC transporter, periplasmic component YrbD (Pseudomonas fluorescens FW300-N2E2)
MQNRTLEIGVGLFLLAGILALLLLALRVSGLAPTASTDTYKLYANFDNIAGLTVRAKVTM
AGVTIGKVTAIDLNREDFTGRVTLQLEKRVDNLPTDSTASILTAGLLGEKYIGISVGGED
SVLKDGGVIHDTQSSLVLEDLIGKFLLNTVSKDAK