Protein Info for Pf6N2E2_3199 in Pseudomonas fluorescens FW300-N2E2

Annotation: S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 349 TIGR00113: S-adenosylmethionine:tRNA ribosyltransferase-isomerase" amino acids 1 to 337 (337 residues), 492 bits, see alignment E=4.3e-152 PF02547: Queuosine_synth" amino acids 4 to 336 (333 residues), 449.1 bits, see alignment E=4.3e-139

Best Hits

Swiss-Prot: 94% identical to QUEA_PSEPF: S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K07568, S-adenosylmethionine:tRNA ribosyltransferase-isomerase [EC: 5.-.-.-] (inferred from 99% identity to pba:PSEBR_a915)

MetaCyc: 71% identical to tRNA preQ134 S-adenosylmethionine ribosyltransferase-isomerase (Escherichia coli K-12 substr. MG1655)
RXN0-1342 [EC: 2.4.99.17]

Predicted SEED Role

"S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 5.-.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.-.-.-

Use Curated BLAST to search for 2.4.99.17 or 5.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165ZE02 at UniProt or InterPro

Protein Sequence (349 amino acids)

>Pf6N2E2_3199 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) (Pseudomonas fluorescens FW300-N2E2)
MRVADFTFELPDSLIARHPLAERRASRLLTLDGVSGAMAHRQFTDLLEHLRPGDLMVFNN
TRVIPARLFGQKASGGKLEILVERVLDSHRVLAHVRSSKSPKPGSSILIDGGGEAVMVAR
HDALFELEFAEEVLPLLDRVGHMPLPPYIDRPDEGSDRERYQTVYAERLGAVAAPTAGLH
FDQPLLEAIAAKGVETAFVTLHVGAGTFQPVRVERIEDHHMHNEWLEVSQEVVDAVAACR
ARGGRVVAVGTTSVRSLESAARDGVLKPFSGDTDIFIYPGRPFHVVDALVTNFHLPESTL
LMLVSAFAGYPEAMAAYKAAVEHGYRFFSYGDAMFITRNPAPRGPEETV