Protein Info for Pf6N2E2_3118 in Pseudomonas fluorescens FW300-N2E2

Annotation: Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 336 PF00072: Response_reg" amino acids 3 to 102 (100 residues), 59.9 bits, see alignment E=2.5e-20 PF01339: CheB_methylest" amino acids 154 to 330 (177 residues), 192.5 bits, see alignment E=5.2e-61

Best Hits

Swiss-Prot: 84% identical to CHEB1_PSEPF: Protein-glutamate methylesterase/protein-glutamine glutaminase 1 (cheB1) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K13491, two-component system, chemotaxis family, response regulator WspF [EC: 3.1.1.61] (inferred from 97% identity to pba:PSEBR_a1021)

Predicted SEED Role

"Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61)" in subsystem Bacterial Chemotaxis (EC 3.1.1.61)

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.61

Use Curated BLAST to search for 3.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZYL6 at UniProt or InterPro

Protein Sequence (336 amino acids)

>Pf6N2E2_3118 Chemotaxis response regulator protein-glutamate methylesterase CheB (EC 3.1.1.61) (Pseudomonas fluorescens FW300-N2E2)
LKIAIVNDMPLAVEALRRALAFEPAHQLVWVAANGAEAVQRCAEYTPDLILMDLLMPIMD
GVEATRRIMADSPCAIVIVTGDSQQNVHRVFEAMGHGALDVVDTPALGVGNPADAAAPLL
RKITNIGWLIGERNKHERPAPAAQRAAVSRKSLVAIGSSAGGPAALEVLLKALPRDFAPA
IVLVQHVDEVFAAGMAEWLGSVSGHTVRLARHGETPQSGTVLLAGTNHHLRLLKDGTLAY
TAEPVNEIYRPSIDVFFESVAQYWSGDAVGVLLTGMGRDGAQGLKLMRQQGYVTVAQDQR
SSAVYGMPKAAAAIDAAVHILALDAIAPRLLEIFTP