Protein Info for Pf6N2E2_3103 in Pseudomonas fluorescens FW300-N2E2
Annotation: Phosphoenolpyruvate carboxylase (EC 4.1.1.31)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 93% identical to CAPP_PSEPF: Phosphoenolpyruvate carboxylase (ppc) from Pseudomonas fluorescens (strain Pf0-1)
KEGG orthology group: K01595, phosphoenolpyruvate carboxylase [EC: 4.1.1.31] (inferred from 99% identity to pba:PSEBR_a1033)MetaCyc: 56% identical to phosphoenolpyruvate carboxylase (Escherichia coli K-12 substr. MG1655)
Phosphoenolpyruvate carboxylase. [EC: 4.1.1.31]
Predicted SEED Role
"Phosphoenolpyruvate carboxylase (EC 4.1.1.31)" in subsystem Fermentations: Mixed acid or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.31)
MetaCyc Pathways
- partial TCA cycle (obligate autotrophs) (8/8 steps found)
- ethene biosynthesis V (engineered) (19/25 steps found)
- CO2 fixation into oxaloacetate (anaplerotic) (2/2 steps found)
- mixed acid fermentation (12/16 steps found)
- nitrogen remobilization from senescing leaves (6/8 steps found)
- C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
- C4 photosynthetic carbon assimilation cycle, PEPCK type (9/14 steps found)
- C4 photosynthetic carbon assimilation cycle, NAD-ME type (6/11 steps found)
- formaldehyde assimilation I (serine pathway) (6/13 steps found)
- gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)
KEGG Metabolic Maps
- Carbon fixation in photosynthetic organisms
- Pyruvate metabolism
- Reductive carboxylate cycle (CO2 fixation)
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.1.1.31
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A159ZWZ2 at UniProt or InterPro
Protein Sequence (878 amino acids)
>Pf6N2E2_3103 Phosphoenolpyruvate carboxylase (EC 4.1.1.31) (Pseudomonas fluorescens FW300-N2E2) MLMTDIDARLREDVHLLGELLGNTIREQYGDAFLDKIEQIRKGAKADRRGSVDAELSASL NQLSEDELLPVARAFNQFLNLANIAEQYQLIHRREESQPEPFEARVLPELLARLRAEGHG AEALARQLGRLEIELVLTAHPTEVARRTLIQKYDAIAAQLAAQDHRDLTSAERAQIHERL QRLIAEAWHTEEIRRTRPTPVDEAKWGFAVIEHSLWQAIPNYLRKADQALHAATGLRLPL EAAPIRFASWMGGDRDGNPNVTAAVTREVLLLARWMAADLYLRDVDQLAAELSMQHASEA LRAQAGDSAEPYRAVLKQLRERLRATRNWAHASLSAATPPSADVLQHNRELLDPLELCYH SLHECGMGVIADAPLLDCLRRAVTFGLFLVRLDVRQDSTRHTAAMTEITDYLGLGRYEDW NEEQRISFLLAELSNRRPLLPAHFKPSADTAEVLATCREVAAAPAASLGSYVISMAGAAS DVLAVQLLLKDAGVLRPMRVVPLFETLADLDNAGPVIERLLLLPGYRSRLQGPQEVMIGY SDSAKDAGTTAAAWAQYRAQERLVDICREQQVELLLFHGRGGTVGRGGGPAHAAILSQPP GSVAGRFRTTEQGEMIRFKFGLPDIAEQNLNLYLAAVLEATLLPPPPPTPQWRDLMDELA ADGVRAYRAVVRENPQFVEYFRQSTPEQELGRLPLGSRPAKRRAGGIESLRAIPWIFGWT QTRLMLPAWLGWETALSKALERGEGELLGQMREQWPFFRTRIDMLEMVLAKADADIARSY DERLVEPGLLPLGAHLRDLLSQACSVVLGLTGQSQLLAHSPATLEFIRLRNTYLDPLHLL QAELLARSRQQDVAQGSPVEQALLVSVAGIAAGLRNTG