Protein Info for Pf6N2E2_3050 in Pseudomonas fluorescens FW300-N2E2
Annotation: CTP synthase (EC 6.3.4.2)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 97% identical to PYRG_PSEFS: CTP synthase (pyrG) from Pseudomonas fluorescens (strain SBW25)
KEGG orthology group: K01937, CTP synthase [EC: 6.3.4.2] (inferred from 100% identity to pba:PSEBR_a1083)MetaCyc: 69% identical to CTP synthetase (Escherichia coli K-12 substr. MG1655)
CTP synthase. [EC: 6.3.4.2]
Predicted SEED Role
"CTP synthase (EC 6.3.4.2)" (EC 6.3.4.2)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
- superpathway of pyrimidine ribonucleotides de novo biosynthesis (9/9 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- superpathway of pyrimidine nucleobases salvage (4/4 steps found)
- L-glutamate and L-glutamine biosynthesis (6/7 steps found)
- UTP and CTP de novo biosynthesis (3/3 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- UTP and CTP dephosphorylation I (5/7 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- UTP and CTP dephosphorylation II (1/3 steps found)
- superpathway of pyrimidine ribonucleosides salvage (6/10 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.4.2
Use Curated BLAST to search for 6.3.4.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A165ZCS1 at UniProt or InterPro
Protein Sequence (543 amino acids)
>Pf6N2E2_3050 CTP synthase (EC 6.3.4.2) (Pseudomonas fluorescens FW300-N2E2) MTRYIFVTGGVVSSLGKGIASASLAAILEARGLKVTMLKLDPYINVDPGTMSPFQHGEVF VTHDGAETDLDLGHYERFIRTTMTQNNNFTTGRVYEHVLRKERRGDYLGATIQVIPHITD EIKRRIIKGAGDADVAMVEIGGTVGDIESQPFLEAIRQLRFEIGAKRAMLMHLTLVPYIA TAGETKTKPTQHSVKELRSIGLQPDVLICRSDHPIDISSRRKIAQFTNVEERAVIGLEDA DTIYKIPGILHSQGLDDFVVERFGLQCASADLSEWEAVVDAKLNPEHEVTIAMVGKYMEL LDAYKSLIEAMSHAGISNRTKVNLRYIDSEDIENQGTGLLEGADAILVPGGFGLRGVEGK ITAVQYARENKVPYLGICLGMQVAVIEFARNVLGWKDANSTEFDRASGHPVVGLITEWED ATGAVEVRTESSDLGGTMRLGAQECLLEAGSKVHDCYAKDVIVERHRHRYEVNNNLLPQL IEAGLKISGRSGDGALVEVVEAPDHPWFVACQFHPEFTSTPRDGHPLFSGFVKAALAQHQ KKA