Protein Info for Pf6N2E2_3048 in Pseudomonas fluorescens FW300-N2E2

Annotation: Enolase (EC 4.2.1.11)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03952: Enolase_N
  • amino acids 4 to 134 (131 residues), 192 bits, E=4.5e-61
• TIGR01060: phosphopyruvate hydratase
  • amino acids 4 to 427 (424 residues), 672.4 bits, E=1.1e-206
• PF00113: Enolase_C
  • amino acids 144 to 426 (283 residues), 458.1 bits, E=1.4e-141

Best Hits

Swiss-Prot: 98% identical to ENO_PSEPF: Enolase (eno) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01689, enolase [EC: 4.2.1.11] (inferred from 99% identity to pba:PSEBR_a1085)

MetaCyc: 74% identical to enolase (Escherichia coli K-12 substr. MG1655)
Phosphopyruvate hydratase. [EC: 4.2.1.11]

Predicted SEED Role

"Enolase (EC 4.2.1.11)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Serine-glyoxylate cycle (EC 4.2.1.11)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of glucose and xylose degradation (16/17 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• Bifidobacterium shunt (14/15 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
• heterolactic fermentation (15/18 steps found)
• chorismate biosynthesis I (7/7 steps found)
• Rubisco shunt (9/10 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
• gluconeogenesis I (11/13 steps found)
• Entner-Doudoroff pathway I (8/9 steps found)
• Entner-Doudoroff pathway III (semi-phosphorylative) (8/9 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• glycolysis III (from glucose) (9/11 steps found)
• ethene biosynthesis V (engineered) (19/25 steps found)
• glycolysis IV (8/10 steps found)
• superpathway of chorismate metabolism (43/59 steps found)
• glycolysis I (from glucose 6-phosphate) (10/13 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (7/9 steps found)
• homolactic fermentation (9/12 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• gallate biosynthesis (2/3 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• gluconeogenesis III (8/12 steps found)
• glycolysis VI (from fructose) (7/11 steps found)
• superpathway of hexitol degradation (bacteria) (12/18 steps found)
• quinate degradation I (1/3 steps found)
• quinate degradation II (1/3 steps found)
• glycerol degradation to butanol (9/16 steps found)
• superpathway of N-acetylneuraminate degradation (13/22 steps found)
• formaldehyde assimilation I (serine pathway) (6/13 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (15/35 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (10/42 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Glycolysis / Gluconeogenesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.11

Sequence Analysis Tools

See A0A159ZX10 at UniProt or InterPro

Protein Sequence (429 amino acids)

>Pf6N2E2_3048 Enolase (EC 4.2.1.11) (Pseudomonas fluorescens FW300-N2E2)
MAKIVDIKGREVLDSRGNPTVEADVLLDNGIIGSACAPSGASTGSREALELRDGDKSRYL
GKGVLKAVANINGPIRDLLLGKDPADQKALDHAMIKLDGTENKASLGANAILAVSLAAAK
AAAQDQDLPLYAHIANLNGTPGVYSMPVPMMNIINGGEHADNNVDIQEFMVQPVGAKTFS
EGLRMGTEIFHHLKAVLKARGLSTAVGDEGGFAPNLASNEDALKVISEAVANAGYKLGTD
VTLALDCAASEFFEDGKYNLSGEGQVFTAEGFADYLKGLTERYPIISIEDGLDESDWAGW
KILTDKIGEKTQLVGDDLFVTNTKILKEGIDKKIANSILIKFNQIGTLTETLEAIQMAKA
AGYTAVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSDRVSKYNQLLRIEEQLNGKAK
YNGRAEFRG