Protein Info for Pf6N2E2_3041 in Pseudomonas fluorescens FW300-N2E2
Annotation: tRNA pseudouridine 13 synthase (EC 4.2.1.-)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 92% identical to TRUD_PSEPF: tRNA pseudouridine synthase D (truD) from Pseudomonas fluorescens (strain Pf0-1)
KEGG orthology group: K06176, tRNA pseudouridine synthase D [EC: 5.4.99.12] (inferred from 97% identity to pba:PSEBR_a1092)Predicted SEED Role
"tRNA pseudouridine 13 synthase (EC 4.2.1.-)" in subsystem tRNA processing (EC 4.2.1.-)
KEGG Metabolic Maps
- 1- and 2-Methylnaphthalene degradation
- Benzoate degradation via CoA ligation
- Benzoate degradation via hydroxylation
- Biosynthesis of type II polyketide backbone
- Biosynthesis of unsaturated fatty acids
- Butanoate metabolism
- Fatty acid biosynthesis
- Limonene and pinene degradation
- Nucleotide sugars metabolism
- Phenylalanine, tyrosine and tryptophan biosynthesis
- Porphyrin and chlorophyll metabolism
- Tyrosine metabolism
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.-, 5.4.99.12
Use Curated BLAST to search for 4.2.1.- or 5.4.99.12
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160A0S9 at UniProt or InterPro
Protein Sequence (352 amino acids)
>Pf6N2E2_3041 tRNA pseudouridine 13 synthase (EC 4.2.1.-) (Pseudomonas fluorescens FW300-N2E2) MTELELLGPRAYGEPLGSAVLKATAEDFQVDEVLDIPLTGDGEHLWIWVEKRGLNTEEAA RRIAKAAGVPLRTVSYAGLKDRQALTRQWFSIQLPGKADPDLSAAENDTLKILKTARHRR KLQRGAHSANGFTLRLTQFNGDTTAIDARLQQIVRQGIPNYFGAQRFGHDGGNVVDARAW AARKALPEQRNVRSRLLSTARSFLFNRVLAARVADGTWQQAQVGDLLAFTDSRSFFPAGE AECSDPRLAILDLHPTGPQWGEGPSPAAGATFELEQAIAAGEADLRDWLINAGMSHERRI LRLPIGGLSWHYPQPDILQLEFVLPAGCFATVLVRELVELVPVGQTDSPCVF