Protein Info for Pf6N2E2_2972 in Pseudomonas fluorescens FW300-N2E2

Annotation: Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00409: proline--tRNA ligase
  • amino acids 1 to 566 (566 residues), 807.1 bits, E=5.2e-247
• PF00587: tRNA-synt_2b
  • amino acids 96 to 457 (362 residues), 116.1 bits, E=3e-37
• PF04073: tRNA_edit
  • amino acids 257 to 374 (118 residues), 86.2 bits, E=2.9e-28
• PF03129: HGTP_anticodon
  • amino acids 473 to 567 (95 residues), 54.9 bits, E=1.2e-18

Best Hits

Swiss-Prot: 96% identical to SYP_PSEPF: Proline--tRNA ligase (proS) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 98% identity to pba:PSEBR_a1142)

MetaCyc: 67% identical to proline--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]; Proline--tRNA ligase. [EC: 6.1.1.16, 6.1.1.15]; 6.1.1.15 [EC: 6.1.1.16, 6.1.1.15]; 3.1.1.M26 [EC: 6.1.1.16, 6.1.1.15, 3.1.1.M26]

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

• tRNA charging (20/21 steps found)
• pyoluteorin biosynthesis (1/7 steps found)
• thiocoraline biosynthesis (1/7 steps found)
• ergotamine biosynthesis (4/12 steps found)
• anguibactin biosynthesis (1/8 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• clorobiocin biosynthesis (1/10 steps found)
• pyochelin biosynthesis (1/11 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• pyrrolomycin biosynthesis (1/14 steps found)
• sulfazecin biosynthesis (2/16 steps found)
• phosalacine biosynthesis (6/25 steps found)
• phosphinothricin tripeptide biosynthesis (5/25 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.16

Use Curated BLAST to search for 3.1.1.M26 or 6.1.1.15 or 6.1.1.16

Sequence Analysis Tools

See A0A160A0M9 at UniProt or InterPro

Protein Sequence (571 amino acids)

>Pf6N2E2_2972 Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type (Pseudomonas fluorescens FW300-N2E2)
MRTSQYLLATQKETPSDAVVISHQLMLRAGMIRKLASGLYTWLPMGLRVMRKVEAIVREE
MDAAGSLEVLMPSTQPAELWQESGRWEEYGPELLRIKDRHGRDFCAGPTHEEVITDLMRN
ELSSYKQLPINLYQIQTKFRDEIRPRFGLMRGREFIMKDAYSFHTDLASLQVTYDRMHQA
YCNVFTRLGLKFRPVEADNGSIGGAGSHEFHVLAESGEDDIVFSNGSDYAANIEKAEAVP
RETSRAAPTEELRLVDTPNAKTIAQLVEGYNLPIERTIKTLIVRAEEAGKLIALIIRGDH
ELNEIKAANQPGVASPLVMATEAELRDAIGAGAGSLGPLNLPLPIIIDRSVELMSDFAIG
ANIDDKHYFGVNWERDLPVPTVADLRNVVAGDPSPDGKGTLEIKRGIEVGHIFQLGNKYS
KAMKCEVLGENGKPVTLEMGCYGIGVSRVVAAAIEQNNDEKGIIWSDTLAPFQIALVPLR
YETDLVREATDKLYAELTAAGFEVLLDDRDKKTSPGIKFADMELIGIPHRIVVSDRGLAE
GNLEYKSRTEAEPQALPVADVLSFLQARIRR