Protein Info for Pf6N2E2_2942 in Pseudomonas fluorescens FW300-N2E2

Annotation: ATP-dependent helicase DinG/Rad3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 714 transmembrane" amino acids 472 to 492 (21 residues), see Phobius details PF00270: DEAD" amino acids 58 to 117 (60 residues), 24.8 bits, see alignment 1.6e-09 PF13307: Helicase_C_2" amino acids 552 to 708 (157 residues), 147.3 bits, see alignment E=4.7e-47

Best Hits

KEGG orthology group: K03722, ATP-dependent DNA helicase DinG [EC: 3.6.4.12] (inferred from 100% identity to pba:PSEBR_a1168)

Predicted SEED Role

"ATP-dependent helicase DinG/Rad3" in subsystem DNA repair, bacterial DinG and relatives

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160A0L2 at UniProt or InterPro

Protein Sequence (714 amino acids)

>Pf6N2E2_2942 ATP-dependent helicase DinG/Rad3 (Pseudomonas fluorescens FW300-N2E2)
MISTELKTTIQGAYSRFLEAKSLKPRYGQRLMIAEVAKVLGDIDTDDEGRRSGEPAVVAV
EAGTGTGKTVAYSLAAIPTAKAAGKRLVIATATVALQEQIVYKDLPDLMRNSGLNFTFAL
AKGRGRYMCLSKLDMLLQEGHAQTATAQLFEEEGFKIEVDEASQKLFTSMIEKLAGNKWD
GDRDSWSTALEDADWSRLTTDHSQCTNRHCPNFGQCAFYKAREGMGKVDVIVTNHDMVLA
DLALGGGAVLPDPRDTIYVFDEGHHLPDKAIGHFAHYTRLRSTADWLETTAKNLTKLLAQ
HPLPGDLGKFIEQVPELAREIKTNQQFMFTACEQVADFKPGEDVEGRERPRHRFVGGVIP
EHMREMGIELKKGFARLTDLFTRLTDLLKEGMDGEVNIGIASNQAEEWYPLFGSLLSRSQ
GNWELWTAFTAEDPEDNPPMARWLTLAESGSLFDIEVNASPILAAEMLRRNLWNVAYGAL
VTSATLTALGTFDRFRMRAGLPKKAVTAVVPSPFHHADAGVLRVPDLKADPRDAAAHTAA
IIRDLPELVEGSRGTLVLFSSRKQMQDVFDGLDRDWRKQVFIQGNLSKQETLNKHKARVD
GGDSSVLFGLASFAEGVDLPGAYCEHVVIAKIPFSVPDDPVEAALAEWIEARGGNPFMEI
SVPDASLKLVQACGRLLRTEEDRGTITLLDRRLVTQRYGKAILNALPPFRREIS