Protein Info for Pf6N2E2_2904 in Pseudomonas fluorescens FW300-N2E2

Annotation: Hypothetical protein DUF454

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 146 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 37 to 46 (10 residues), see Phobius details amino acids 79 to 97 (19 residues), see Phobius details amino acids 102 to 119 (18 residues), see Phobius details PF04304: DUF454" amino acids 10 to 123 (114 residues), 119 bits, see alignment E=5.4e-39

Best Hits

KEGG orthology group: K09790, hypothetical protein (inferred from 94% identity to pba:PSEBR_a1202)

Predicted SEED Role

"Hypothetical protein DUF454" in subsystem Heme, hemin uptake and utilization systems in GramPositives

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165ZBR3 at UniProt or InterPro

Protein Sequence (146 amino acids)

>Pf6N2E2_2904 Hypothetical protein DUF454 (Pseudomonas fluorescens FW300-N2E2)
MSRPSKLTQLLFALLAYASLGIGLVAIVVPGLPTTEFILLAAWAATRSSPRLSAWLENHR
LFGPILHNWRNGKVIPRRAKVGATTSMLLCAGLMLVLLDHAWPLYLALAGMGLGNLWIWS
RPEQARGSCGEGACSRWAAKRPPTDF