Protein Info for Pf6N2E2_2870 in Pseudomonas fluorescens FW300-N2E2

Annotation: Ribonuclease BN (EC 3.1.-.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 316 transmembrane" amino acids 35 to 58 (24 residues), see Phobius details amino acids 94 to 115 (22 residues), see Phobius details amino acids 136 to 158 (23 residues), see Phobius details amino acids 179 to 202 (24 residues), see Phobius details amino acids 211 to 234 (24 residues), see Phobius details amino acids 245 to 267 (23 residues), see Phobius details TIGR00765: YihY family inner membrane protein" amino acids 18 to 275 (258 residues), 147.7 bits, see alignment E=2.7e-47 PF03631: Virul_fac_BrkB" amino acids 24 to 277 (254 residues), 240.2 bits, see alignment E=1.5e-75

Best Hits

KEGG orthology group: K07058, membrane protein (inferred from 98% identity to pba:PSEBR_a1233)

Predicted SEED Role

"Ribonuclease BN (EC 3.1.-.-)" in subsystem LMPTP YfkJ cluster or tRNA processing (EC 3.1.-.-)

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZWH8 at UniProt or InterPro

Protein Sequence (316 amino acids)

>Pf6N2E2_2870 Ribonuclease BN (EC 3.1.-.-) (Pseudomonas fluorescens FW300-N2E2)
MMFPALKGLPLHRVMMRTVTEFLDDEMSTYASALAYQMLFSLFPFILFLIALIGFLHLPD
FFSWLRLQSELVLPPQALEQVNPVIDQLQQSKGGLLSVGIVIALWTASAGVRLMMSAMNA
AYDVVEGRPAWKRFPLSIFYTVGIAGMLLAAAALMVLGPQVMGWIAAQVGLEEFIVTLWT
IVRWPVIVILLMVAVALIYYVMPDVKQEFRFITPGSVLAVVVWIIASLGFAFYVKTFANY
NAMYGSIGAIIVLLLYFYISSAVLLLGAEMNAVIEHMSTEGKNPGEKSAGEHEKQHVSGL
GRDHSIPHPHPDEARP