Protein Info for Pf6N2E2_2823 in Pseudomonas fluorescens FW300-N2E2

Annotation: Multidrug efflux RND membrane fusion protein MexC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 385 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF00529: CusB_dom_1" amino acids 35 to 345 (311 residues), 54 bits, see alignment E=2.7e-18 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 37 to 359 (323 residues), 264.1 bits, see alignment E=7.5e-83 PF16576: HlyD_D23" amino acids 61 to 277 (217 residues), 55.4 bits, see alignment E=1.1e-18 PF13533: Biotin_lipoyl_2" amino acids 62 to 109 (48 residues), 30.1 bits, see alignment 6.5e-11 PF13437: HlyD_3" amino acids 159 to 274 (116 residues), 30.6 bits, see alignment E=9.3e-11

Best Hits

Swiss-Prot: 78% identical to MEPA_PSEPU: Multidrug/solvent efflux pump periplasmic linker protein MepA (mepA) from Pseudomonas putida

KEGG orthology group: K03585, membrane fusion protein (inferred from 98% identity to pba:PSEBR_a1278)

MetaCyc: 57% identical to multidrug efflux pump membrane fusion lipoprotein AcrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN-360; TRANS-RXN0-592

Predicted SEED Role

"Multidrug efflux RND membrane fusion protein MexC" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165ZB88 at UniProt or InterPro

Protein Sequence (385 amino acids)

>Pf6N2E2_2823 Multidrug efflux RND membrane fusion protein MexC (Pseudomonas fluorescens FW300-N2E2)
MQFKPAVTALVTAIALASLLSGCKKEEAAPAPPPPQVGVVTLKTQPFTLTSELPGRTSAF
RVAEVRPQVNGIILKRLFKEGADVKAGQQLYQIDPAVYEATLKSAEANLRSTKSIADRYK
QLVDEQAVSRQEYDTAVANRLESEANLQTAQINVRYTKVYAPISGRIGRSSVTEGALVSN
GQTDALATIQQLDPIYVDVTQSSVELLQLRRELESGRLQKAGDNAAMVKLTLEDGSLYPH
EGKLEFSEVSVDQTTGSVTLRAVFPNPDHTLLPGMFVHAQLQAGVNSAAILAPQQGVTRD
LKGTPTALVVGADNKVELRQLKASRTVGSQWLIEDGLKAGDRVITEGLQFVRPGVEVKAT
EATNIDTKNPAPAQAADKAAGGKGE