Protein Info for Pf6N2E2_2778 in Pseudomonas fluorescens FW300-N2E2

Annotation: Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03425: urea carboxylase-associated protein 2
  • amino acids 12 to 240 (229 residues), 321.2 bits, E=1.6e-100
• PF09347: DUF1989
  • amino acids 15 to 186 (172 residues), 182.7 bits, E=2.4e-58

Best Hits

KEGG orthology group: K09967, hypothetical protein (inferred from 98% identity to pba:PSEBR_a1320)

Predicted SEED Role

"Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10)" in subsystem Urea decomposition (EC 2.1.2.10)

MetaCyc Pathways

• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Nitrogen metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.10

Use Curated BLAST to search for 2.1.2.10

Sequence Analysis Tools

See A0A159ZXX0 at UniProt or InterPro

Protein Sequence (241 amino acids)

>Pf6N2E2_2778 Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10) (Pseudomonas fluorescens FW300-N2E2)
MTDSTHLFTPFAEELLPGGGHRSFVLKRGQLLRLTDLRGGANVSLTLLNANEKTERLNLP
DSLKCQHTAKLTAGHCLYSDMGRVLAAITADTCGWSDSLGGVLCAEEVAQKYGQGRYQEL
RNGFFRNGTDNLLVELGKWGLGLSDLLMTLNLFSRVDVDEAGNFHFVEGNSLTGDYIELY
APMDTLVVLTALQHPMDPNPQYAPQPLKLSWMNADASVAEHCRLSRPENQRGFINTDRLF
A