Protein Info for Pf6N2E2_2725 in Pseudomonas fluorescens FW300-N2E2

Annotation: D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02826: 2-Hacid_dh_C
  • amino acids 103 to 274 (172 residues), 145.1 bits, E=1.5e-46
• PF03446: NAD_binding_2
  • amino acids 137 to 225 (89 residues), 21.6 bits, E=1.9e-08

Best Hits

KEGG orthology group: None (inferred from 99% identity to pba:PSEBR_a1368)

Predicted SEED Role

"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• L-serine biosynthesis I (3/3 steps found)
• L-cysteine biosynthesis IX (Trichomonas vaginalis) (2/3 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.95

Use Curated BLAST to search for 1.1.1.95

Sequence Analysis Tools

See A0A160A055 at UniProt or InterPro

Protein Sequence (310 amino acids)

>Pf6N2E2_2725 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) (Pseudomonas fluorescens FW300-N2E2)
MRVLIAEHDHPVYAQLLRQAAPDIEVLTSGDSAELSRLATDCPVWLGQPDLLATLLRQGH
RPSWLQSTWAGITPLLAEGLTRNYRLTRAVGIFGQVMAEYVLTYMLGHEREVLARLVSQV
ERKWDNRQGQGLAGRKVLIVGTGDIGQRVAQFLVPFGVQLYGVASEARALAPFIEVGALK
DLPRLVGEADYVINLLPNTPDTHDVYDAALFKQFKPTGLFINVGRGVAVVDADLVQALKD
GHLAGAVIDVCRQEPLPQRHPFWTAWGLLLTGHSSAPTSPPMMVQLFLENLRAYQAGEAL
RGEVDFSRGY