Protein Info for Pf6N2E2_2619 in Pseudomonas fluorescens FW300-N2E2

Annotation: Glucose-1-phosphate cytidylyltransferase (EC 2.7.7.33)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00483: NTP_transferase
  • amino acids 1 to 221 (221 residues), 61.3 bits, E=1.1e-20
• TIGR02623: glucose-1-phosphate cytidylyltransferase
  • amino acids 1 to 251 (251 residues), 448.7 bits, E=2.8e-139

Best Hits

Swiss-Prot: 66% identical to RFBF_SALTI: Glucose-1-phosphate cytidylyltransferase (rfbF) from Salmonella typhi

KEGG orthology group: K00978, glucose-1-phosphate cytidylyltransferase [EC: 2.7.7.33] (inferred from 93% identity to ppw:PputW619_3719)

MetaCyc: 66% identical to alpha-D-glucose-1-phosphate cytidylyltransferase monomer (Yersinia pseudotuberculosis)
Glucose-1-phosphate cytidylyltransferase. [EC: 2.7.7.33]

Predicted SEED Role

"Glucose-1-phosphate cytidylyltransferase (EC 2.7.7.33)" in subsystem dTDP-rhamnose synthesis (EC 2.7.7.33)

MetaCyc Pathways

• CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis (3/3 steps found)
• CDP-6-deoxy-D-gulose biosynthesis (3/5 steps found)
• superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis (3/8 steps found)
• streptomycin biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Nucleotide sugars metabolism
• Starch and sucrose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.33

Sequence Analysis Tools

See A0A159ZW57 at UniProt or InterPro

Protein Sequence (252 amino acids)

>Pf6N2E2_2619 Glucose-1-phosphate cytidylyltransferase (EC 2.7.7.33) (Pseudomonas fluorescens FW300-N2E2)
LAGGLGTRISEESHLKPKPMIEIGGKPILWHIMKQYSAHGIHDFVICLGYKGYAIKDFFA
NYFLHTSDVTFDMCNNRMDVHQNYSEPWRVTLIDTGEDTMTGGRLRRAARYLENEEAFCF
TYGDGVSDLNIGALVDFHLSHGKLATVTAVQPPGRYGALERDGDTVLGFTEKPRGDGGWI
NGGFFVLSPKVLPYIADDQTSWEAEPLAELATEQQLQAFQHDGFWHPMDTLRDKNHLEAL
WQSGEAPWKQWD