Protein Info for Pf6N2E2_2607 in Pseudomonas fluorescens FW300-N2E2

Annotation: N-acetylneuraminate synthase (EC 2.5.1.56)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 TIGR03586: pseudaminic acid synthase" amino acids 16 to 344 (329 residues), 499.9 bits, see alignment E=1.7e-154 PF03102: NeuB" amino acids 37 to 278 (242 residues), 292.2 bits, see alignment E=3e-91 PF08666: SAF" amino acids 292 to 350 (59 residues), 39.5 bits, see alignment E=7e-14

Best Hits

Swiss-Prot: 46% identical to PSEI_CAMJE: Pseudaminic acid synthase (pseI) from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

KEGG orthology group: K01654, N-acetylneuraminate synthase [EC: 2.5.1.56] (inferred from 89% identity to pfo:Pfl01_1523)

MetaCyc: 46% identical to pseudaminic acid synthase (Campylobacter jejuni)
RXN-10002 [EC: 2.5.1.97]

Predicted SEED Role

"N-acetylneuraminate synthase (EC 2.5.1.56)" in subsystem CMP-N-acetylneuraminate Biosynthesis or Sialic Acid Metabolism (EC 2.5.1.56)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.56 or 2.5.1.97

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A165Z9T7 at UniProt or InterPro

Protein Sequence (350 amino acids)

>Pf6N2E2_2607 N-acetylneuraminate synthase (EC 2.5.1.56) (Pseudomonas fluorescens FW300-N2E2)
MASFKIGHCVIGAEAPPFIIAEMSGNHNQSLEVALEIVEAAAKAGAHALKLQTYTAETMT
LDLNEGEFFIKDPNSLWAGTSLYELYEKAHTPWEWHAPIFARAKALGMLAFSTPFDETAV
DFLEALDVPAYKIASFENTDLPLIRRVAATGKPLIISTGMASVAELDETVRAAREAGCKD
LVLLKCTSTYPATPANSNVRTIPHLRELFGCEVGLSDHSMGVGVSVAAVALGATVVEKHF
TLDRAAGGVDASFSLEPAELAALVIETERAWQAMGQVHYGVTEAERKSLVYRRSLYVTQD
MSPGDAFTAQNVRAIRPGLGLPPKYADSLMGRCARQAIKRGTPLAWSLVE