Protein Info for Pf6N2E2_2493 in Pseudomonas fluorescens FW300-N2E2

Annotation: Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 684 transmembrane" amino acids 45 to 65 (21 residues), see Phobius details amino acids 71 to 92 (22 residues), see Phobius details amino acids 223 to 245 (23 residues), see Phobius details amino acids 256 to 280 (25 residues), see Phobius details amino acids 589 to 609 (21 residues), see Phobius details amino acids 618 to 637 (20 residues), see Phobius details amino acids 657 to 680 (24 residues), see Phobius details TIGR01497: K+-transporting ATPase, B subunit" amino acids 23 to 679 (657 residues), 968.8 bits, see alignment E=1.3e-295 TIGR01494: HAD ATPase, P-type, family IC" amino acids 79 to 348 (270 residues), 108.2 bits, see alignment E=3.7e-35 amino acids 373 to 604 (232 residues), 148.8 bits, see alignment E=1.7e-47 PF00122: E1-E2_ATPase" amino acids 114 to 288 (175 residues), 101.7 bits, see alignment E=7e-33 PF00702: Hydrolase" amino acids 307 to 532 (226 residues), 87.8 bits, see alignment E=2.5e-28 PF12710: HAD" amino acids 445 to 528 (84 residues), 28.9 bits, see alignment E=3e-10

Best Hits

Swiss-Prot: 86% identical to KDPB_PSEAE: Potassium-transporting ATPase ATP-binding subunit (kdpB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01547, K+-transporting ATPase ATPase B chain [EC: 3.6.3.12] (inferred from 98% identity to pba:PSEBR_a1587)

Predicted SEED Role

"Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1)" in subsystem Potassium homeostasis (EC 3.6.3.12, TC 3.A.3.7.1)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.12

Use Curated BLAST to search for 3.6.3.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZZM3 at UniProt or InterPro

Protein Sequence (684 amino acids)

>Pf6N2E2_2493 Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1) (Pseudomonas fluorescens FW300-N2E2)
MNMPMSKTVAAKAPEQPKTHLSALWRPALVQAFVKLDPRQLHRAPVMLVVELTAILTTVL
CFIPDSAVPTFVAAQIALWLWFTVLFANFAEALAEGRGKARADSLKAGSEGLNARRQTAS
GFEIIPATRLRKGDVVRVEAGDMIPGDGEVIEGIAAVNEAAITGESAPVIRESGGDRSAV
TGNTRLVSDWLLIRITSNPGESTLDRMIALVEGAKRQKTPNEVALDILLIGLTLIFLLVV
VTLQPFAHFANANLPLVFLVALLVTLIPTTIGGLLSAIGIAGMDRLVRLNVIAKSGRAVE
AAGDVHVLMLDKTGTITFGNRRCTAVYAAPGVSAKELAEGALFASLADDTAEGKSIVEYL
RGLHPQSEPGVEALTAVPFSAETRLSGVDYQGRIYRKGAVDSLLDFIGLARNELPQALAR
EVDKIAQSGGTPLLVCVDGKLLGAIHLKDVVKPGIRERFAELRKLGIRTVMVTGDNPLTA
AAIAAEAGVDDVLAEATPEKKLARIRHEQNDGRLVAMCGDGANDAPALAQADVGMAMNDG
TQAAREAANMVDLDSDPTKLLDVVQIGKELLVTRGALTTFSIANDIAKYFAILPALFASI
YPQLGVLNVMHLSSPQSAIVSAIVFNALIIVVLIPLALRGVRVQAASAAALLRRNLLIYG
LGGIVVPFVGIKAIDMLLTALHLV