Protein Info for Pf6N2E2_2491 in Pseudomonas fluorescens FW300-N2E2

Annotation: Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 883 transmembrane" amino acids 388 to 413 (26 residues), see Phobius details amino acids 418 to 447 (30 residues), see Phobius details amino acids 466 to 484 (19 residues), see Phobius details PF02702: KdpD" amino acids 19 to 227 (209 residues), 309.1 bits, see alignment E=3.4e-96 PF00582: Usp" amino acids 248 to 360 (113 residues), 31.6 bits, see alignment E=5.9e-11 PF13493: DUF4118" amino acids 390 to 494 (105 residues), 97.7 bits, see alignment E=8.4e-32 PF00512: HisKA" amino acids 652 to 719 (68 residues), 53.3 bits, see alignment 5.7e-18 PF02518: HATPase_c" amino acids 767 to 874 (108 residues), 82.5 bits, see alignment E=7.7e-27

Best Hits

KEGG orthology group: K07646, two-component system, OmpR family, sensor histidine kinase KdpD [EC: 2.7.13.3] (inferred from 99% identity to pba:PSEBR_a1589)

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZVP9 at UniProt or InterPro

Protein Sequence (883 amino acids)

>Pf6N2E2_2491 Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-) (Pseudomonas fluorescens FW300-N2E2)
MSDSGRADALLANLPRNDRGRLKVFLGAAPGVGKTYAMLQAAHTQLRQGVKVVAGVVETH
GRAETEALLGGLVQQPLVRSEYRGVMLEEMDLDGLLAARPALVLVDELAHSNAPGSRHAK
RWQDIQELLAAGIDVYTTVNVQHLESLNDQVRGITGVQVRETLPDWVLQEADELLLIDLP
SRELLERLRDGKVYVPEQARAAIDAFFTQTNLTALRELAMQTAAAQVDNDLAQGYRQLGQ
AAPAVRGRLLVGVGADVQAERLVRHASRVAQRRHLPWSLVHVDSGVAHDESSRQYLQNAQ
QLAERLGGEVVLLRAGEVAKTLIQHATERRATLLLVGQSRPRLRRRLFGGGLASRLLRDA
RGLEINVLDSDEQPRPARVRSNAVQTGFDYVLALTATVAASALAWGISGWLALPNISLVF
LMAVLLVAVRSSLGPALACAALSFLAYDFLFIPPNFSFTIQREEDVLTLVFFLLMAALTG
NLAARQRRQLQALRDTQQETGELLDLSRKLTAATDRQAVISAAAQHLNGLHDLQLCLLNR
DGQRDWKVETGEPLTFTEAERAAADWAWQHDQPAGAGTGTLPSGRWWWWPLSAEGGPLGL
LGVCPNEGKPLSGQRRRLLAALSQPLAQALARAQLAQDLEAARLHGETEQLRSALLASVS
HDLRTPLTSMRGSIDSLLALGEAIPLADRRELLEGTRDEAERLDRYIQNLLDMTRLGHGA
LKLARDWVSPADIVGSALNRLRAVLAPLALSVEVPAELPLLYVHAALIEQALVNVLENAA
RFSPVHGRLLLSAGVTDSEVFFAVADEGPGIPEQERENIFDMFYTAARGDRGGQGTGLGL
AICQGMVGAHGGRISVADGLDGRGTCITLHLPLQAQPGMDDEA