Protein Info for Pf6N2E2_2476 in Pseudomonas fluorescens FW300-N2E2

Annotation: Arylesterase precursor (EC 3.1.1.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00657: Lipase_GDSL
  • amino acids 24 to 191 (168 residues), 79.5 bits, E=4.2e-26
• PF13472: Lipase_GDSL_2
  • amino acids 27 to 185 (159 residues), 112.6 bits, E=3.3e-36

Best Hits

Swiss-Prot: 70% identical to EST_PSEAE: Esterase TesA (tesA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K10804, acyl-CoA thioesterase I [EC: 3.1.1.5 3.1.2.-] (inferred from 100% identity to pba:PSEBR_c2g32)

Predicted SEED Role

"Arylesterase precursor (EC 3.1.1.2)" (EC 3.1.1.2)

MetaCyc Pathways

• phospholipid remodeling (phosphatidylethanolamine, yeast) (2/4 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Bisphenol A degradation
• Fatty acid biosynthesis
• Glycerophospholipid metabolism
• Limonene and pinene degradation
• Ubiquinone and menaquinone biosynthesis
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.5, 3.1.2.-

Use Curated BLAST to search for 3.1.1.2 or 3.1.1.5 or 3.1.2.-

Sequence Analysis Tools

See A0A159ZYG8 at UniProt or InterPro

Protein Sequence (201 amino acids)

>Pf6N2E2_2476 Arylesterase precursor (EC 3.1.1.2) (Pseudomonas fluorescens FW300-N2E2)
MRVWFLSAGLALMCMAQNAAAGTVLIVGDSISAAFGLDTRQGWVSLLEQRLKAEGFDDKV
VNASISGDTSAGGQARLPALLAAHKPDLVILELGGNDGLRGQPPTQLQQNLAAMIDSARA
SGAQVLLLGMQLPPNYGRRYTEAFARVYSTLAEEKKVPLVPFFLKDVGGIPTMMQGDGLH
PSVAAQGKLLENVWPTLKPLL