Protein Info for Pf6N2E2_2437 in Pseudomonas fluorescens FW300-N2E2

Annotation: Hybrid sensory histidine kinase in two-component regulatory system with EvgA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1187 transmembrane" amino acids 520 to 541 (22 residues), see Phobius details PF00497: SBP_bac_3" amino acids 47 to 257 (211 residues), 93 bits, see alignment E=7.3e-30 amino acids 286 to 505 (220 residues), 118.7 bits, see alignment E=9.6e-38 PF00989: PAS" amino acids 561 to 673 (113 residues), 25.8 bits, see alignment E=3.1e-09 PF08448: PAS_4" amino acids 568 to 678 (111 residues), 40.5 bits, see alignment E=1e-13 PF00512: HisKA" amino acids 696 to 762 (67 residues), 60.1 bits, see alignment 6e-20 PF02518: HATPase_c" amino acids 811 to 926 (116 residues), 92.2 bits, see alignment E=1e-29 PF00072: Response_reg" amino acids 950 to 1064 (115 residues), 85 bits, see alignment E=1.5e-27 PF01627: Hpt" amino acids 1095 to 1177 (83 residues), 38.7 bits, see alignment 3.3e-13

Best Hits

KEGG orthology group: K07679, two-component system, NarL family, sensor histidine kinase EvgS [EC: 2.7.13.3] (inferred from 96% identity to pba:PSEBR_a1637)

Predicted SEED Role

"Hybrid sensory histidine kinase in two-component regulatory system with EvgA" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A159ZVK5 at UniProt or InterPro

Protein Sequence (1187 amino acids)

>Pf6N2E2_2437 Hybrid sensory histidine kinase in two-component regulatory system with EvgA (Pseudomonas fluorescens FW300-N2E2)
MLTAAANAPEQYTLLGRSSMAHTEVKLDDVHRAWLQDRQALVLGTSAPDYPPFDLSTSGR
DYEGLTADYADIIAKAIDLPITVLRFGSREAAMAALKDGQIDMLGTANGFEARNPDIVLS
TPYAVDQPVLVTRTNESRSLTDDLEGLRLSMVYHYLPLREIEKLYPKALITTYPSYQNAI
NAVAFGQADVFLGDTLSTHYMINKGYLSNIRMASFGKHESHGFGFAVRRSDTRLLEVINA
TLSQVSIAEQAAISKRWSAGSDLYLTDHRIQLTDREQRWLAGHPVVRVVVNETSAPFTFF
DADGDFRGISADLLELIRLRTGLRLDIQRRQNDSEMIAAVLNDQADMIAALLPSSERQLQ
LKFSRPYVDSSFVLLTRKQSDTPTTLDQLGHASLAIAKGNPVIGWLRSQYPNLQIIETDG
PLRAVEMLAHGQVDGSVNSQVMANYFISSYAWRDRLQISSTVGTQQAMFALATAKNATEL
NAILDKALTSIDPDELGVINNRWRGYVSPSEHTWRTYNRLFLQVVVGVGLLLLFSLAWNA
YMQRQIRQRQRAELALNDQLEFMHSLLNGTPHPIYVRDRNGILQSCNDSYLQTFDVKRED
VIGKSLMPGSMSNAFEAQEYQADYQRVMAEGTALIVDRPLHIGDRQLTIYHWILPYRNSA
AEVQGIIGGWIDISERRQLFEELRASKQRADDANRAKSTFLATMSHEIRTPMNAVIGMLE
LALKQADKGRVDRSTIEVAYESASGMLELIGDILDIARIESGHLSLAPERVNLRALLQSV
IRVFEGVARQKNLALSLQFDAAKGSPDVLMDPLRFKQVLSNLISNALKFTEQGQVVIKVR
LSPTDRDDVLQMHLEVNDSGIGIAPADLQRLFEPFAQVDNTSRMARNGAGLGLVISRNLC
RMMGGSLHMNSQPGTGTQVQVCLQLTVLATAPVRPPPEPVIETAQAPLNVLIVDDHPANR
LLMSQQLEYLGHHYQVAHDGAHGLEVWQENHFDLVIADCNMPIMSGYELARSIRRDESEQ
QRPPCTLLGFTANAQPEEKQRCQDAGMDDCLFKPISLSALSQWIKAVKPVSCPAAFSIQS
LQALTGGDPSSNRRLLAELLSSSRSDRQELLDIAHSANRSALAEIAHKIKGVARIVQATS
LIQRCEALEHACHQAQAPRRISDCIDALDRAMSELERALETEIAKAK