Protein Info for Pf6N2E2_2389 in Pseudomonas fluorescens FW300-N2E2

Annotation: Phosphoenolpyruvate synthase (EC 2.7.9.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01418: phosphoenolpyruvate synthase
  • amino acids 5 to 786 (782 residues), 1213 bits, E=0
• PF01326: PPDK_N
  • amino acids 17 to 345 (329 residues), 427.6 bits, E=5.3e-132
• PF00391: PEP-utilizers
  • amino acids 383 to 454 (72 residues), 97.1 bits, E=5.6e-32
• PF02896: PEP-utilizers_C
  • amino acids 481 to 780 (300 residues), 206.6 bits, E=7.2e-65

Best Hits

Swiss-Prot: 92% identical to PPSA_PSEAB: Phosphoenolpyruvate synthase (ppsA) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 88% identity to avn:Avin_23280)

MetaCyc: 68% identical to phosphoenolpyruvate synthetase (Escherichia coli K-12 substr. MG1655)
Pyruvate, water dikinase. [EC: 2.7.9.2]

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (14/17 steps found)
• gluconeogenesis I (11/13 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• glycolysis I (from glucose 6-phosphate) (10/13 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of hexitol degradation (bacteria) (12/18 steps found)
• superpathway of N-acetylneuraminate degradation (13/22 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See A0A159ZZF5 at UniProt or InterPro

Protein Sequence (791 amino acids)

>Pf6N2E2_2389 Phosphoenolpyruvate synthase (EC 2.7.9.2) (Pseudomonas fluorescens FW300-N2E2)
LVEYVVSLDKLGVHDVEHVGGKNASLGEMISNLAGAGVSVPGGFATTAQAYRDFLELSGL
NKQIHDALDALDVDDVNALAKTGAQIRQWIMEAEFPEKLNAEIRTAFAKLSEGNPDIAVA
VRSSATAEDLPDASFAGQQETFLNIRGVENVIRAAKEVFASLFNDRAISYRVHQGFDHKL
VALSAGVQRMVRSETGTAGVMFTLDTESGFRDVVFITGAYGLGETVVQGAVNPDEFYVHK
GTLQAGRPAILRRNLGSKAIKMIYGDEAKAGRSVKTVDVDKAERARFCLTDAEVSELAKQ
AMIIEQHYKCPMDIEWAKDGDDGKLYIVQARPETVKSRTQANVMERYLLKETGTVLVEGR
AIGQRIGAGKVRIINDVSEMDKVQAGDVLVSDMTDPDWEPVMKRASAIVTNRGGRTCHAA
IIARELGIPAVVGCGNATQLLKDGQGVTVSCAEGDTGYIFEGELGFDIKKNSVDAMPDLP
FKIMMNVGNPDRAFDFAQLPNAGVGLARLEFIINRMIGVHPKALLNYDGLPQDIKDSVDK
RIAGYNDPVGFYVEKLVEGISTLAAAFWPKKVIVRLSDFKSNEYANLIGGKLYEPEEENP
MLGFRGASRYISESFRDCFELECRALKRVRNEMGLTNVEIMVPFVRTLGEASQVVDLLAE
NGLARGDNGLRVIMMCELPSNAILAEEFLEFFDGFSIGSNDLTQLTLGLDRDSGIIAHLF
DERNPAVKKLLANAIAACNKAGKYIGICGQGPSDHPDLAKWLMEQGIESVSLNPDSVLET
WFFLAEGQAAV